Bioactivities |
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Identification |
Melting point |
70°C |
1HNMR
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Analytical Method |
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INSTRUMENT |
Agilent (Waldbronn, Germany) 1100 HPLC system (binary pump, degasser and autosampler) with diode array detector |
COLUMN |
Reversed phase (Phenomenex, Aschaffenburg, Germany 250 × 2 mm i.d., 4 µm, Synergi polar RP) column |
MOBILE PHASE |
A: water, 5 mM ammonium acetate buffer, 0.2% (v/v) acetic acid, B: acetonitrile/methanol 1: 2 (v/v)), 0-2 min 70% A, 2-20 min 70-20% A, 20-22 min 20-70% A, 22-30 min 70% A, 0.2 mL/min |
DETECTION |
UV λ276 nm |
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INSTRUMENT |
Hewlett-Packard (HP) 6890 GC system and HP5973 mass-selective detector (Avondale, PA, USA) |
COLUMN |
30-m DB-5MS column (J&W Scientific, Folsom, CA, USA) |
MOBILE PHASE |
Helium. hold at 55°C for 1 min, ramp at 30°C/min to 110°C, ramp at 3°C/min to 155°C, ramp at 30°C/min to 270°C. Transfer line 280°C, quadrupole 110°C and source heaters 230°C, 1.2 mL/min. |
DETECTION |
SIM mode with a quantitation and a confirmation ion. The dwell time for each SIM scan was 15 ms. |
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Reference |
[1]
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Fentem, J. H., et al. (1992). "Species differences in the hepatotoxicity of coumarin: A comparison of rat and Mongolian gerbil." Toxicology 71(1–2): 129-136. |
[2]
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Stefanova, T., et al. (2007). "Enhanced resistance to Salmonella enterica serovar Typhimurium infection in mice after coumarin treatment." Microbes and Infection 9(1): 7-14. |
[3]
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Sproll, C., et al. (2008). "HPLC analysis and safety assessment of coumarin in foods." Food Chemistry 109(2): 462-469. |
[4]
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Stanfill, S. B. and D. L. Ashley (1999). "Solid phase microextraction of alkenylbenzenes and other flavor-related compounds from tobacco for analysis by selected ion monitoring gas chromatography–mass spectrometry." Journal of Chromatography A 858(1): 79-89. |
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