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植物来源 |
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生物活性 |
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鉴定 |
熔点 |
52-53°C |
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| 分析方法 |
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| 仪器 |
硅胶 G 板 |
| 流动相 |
环己烷: 乙酸乙酯: 丙酮: 甲酸 = 8: 2: 1: 0.2, v/v/v/v |
| 检测器 |
UV λ292 nm |
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| 仪器 |
Waters 600E (USA) HPLC 系统包括四元泵600E 启动组件, 600 泵 (0-20 mL/min), 2996光电二极管数组检测系统, Empower 附加独立系统, 在线4室脱气机, 600E 控制器 |
| 色谱柱 |
C18 色谱柱 (250 mm × 4.6 mm, i.d., 5 µm) |
| 流动相 |
甲醇: 0.5% 甲酸水 = 50: 50, v/v, 1.0 mL/min |
| 检测器 |
UV λ276 nm |
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| 仪器 |
Agilent 1100 series LC 系统 (Agilent Technologies, CA, USA) 包括一个G1312A 二元泵, G1379A 脱气机, G1315B 二极体阵列检测器和G1313A 自动进样器, MicroTOF-Q 质谱仪 (Bruker Daltonics Inc., MA, USA) |
| 色谱柱 |
Henomenex ODS 色谱柱 (250 mm × 4.6 mm i.d.; 粒径5 µm; Phenomenex Inc., USA) 配一个保护柱 (C18, 4 mm × 3.0 mm i.d.; Phenomenex Inc., USA) |
| 流动相 |
A: 乙腈, B: 0.1% 甲酸, 三乙胺调节至 pH 3.5 ± 0.2 : 25-90% = A 0-23 min 流速1.0 mL/min; LC-DAD-MS : 0.05% TFA = A 和含0.05% TFA 50% 乙腈 = B = 0-15% = B 0-20 min, 15-50% = B 20-60 min, 50-100% = B 60-70 min, 和 100% = B 70-80 min 流速1.0 mL/min |
| 检测器 |
电喷雾离子源正负离子模式. MS利用端板偏移电压-500 V, 毛细管电压 4500 V, 毛细管温度 200°C, 干燥气 (N2) 4.0 l/min, 气温 200°C, 雾化气 (N2) 压力 0.4 bar. 50-400 m/z 分辨率 10, 000. |
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| 样品制备 |
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方法一 |
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Model GS-10A 高速逆流色谱 (Beijing Institute of New Technology Application, Beijing, China) 配备一个聚四氟乙烯多层线圈 (i.d. = 1.6 mm, 总容量 = 230 ml) 及一个手动进样阀和 10-ml 回路. Model NS-1007A 恒流泵 (Beijing Institute of New Technology Application, Beijing, China), Model 8823B-UV 检测器 (Beijing Institute of New Technology Application, Beijing, China) BF-2002 CT11 信号收集器 (Chromatography Center of Beifenruili Group Company, Beijing, China) |
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乙醚: 乙酸乙酯: 甲醇: 水 = 2: 6: 3: 4, v/v |
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|
1.7 mL/min, 850 rpm |
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UV λ280 nm |
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| 参考文献 |
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[1]
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Sun, Y., et al. (2008). "Supercritical fluid extraction of paeonol from Cynanchum paniculatum (Bge.) Kitag. and subsequent isolation by high-speed counter-current chromatography coupled with high-performance liquid chromatography-photodiode array detector." Separation and Purification Technology 64(2): 221-226. |
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[2]
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Zhou, J., et al. (2011). "Paeonol increases levels of cortical cytochrome oxidase and vascular actin and improves behavior in a rat model of Alzheimer's disease." Brain Research 1388(0): 141-147. |
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[3]
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Nizamutdinova, I. T., et al. (2007). "Paeonol suppresses intercellular adhesion molecule-1 expression in tumor necrosis factor-α-stimulated human umbilical vein endothelial cells by blocking p38, ERK and nuclear factor-κB signaling pathways." International Immunopharmacology 7(3): 343-350. |
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[4]
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Li, Y.-j., et al. (2010). "Vascular dilation by paeonol — A mechanism study." Vascular Pharmacology 53(3–4): 169-176. |
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[5]
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Ma, Y.-l., et al. (2006). "The effects of paeonol on the electrophysiological properties of cardiac ventricular myocytes." European Journal of Pharmacology 545(2–3): 87-92. |
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[6]
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Zhong, S.-Z., et al. (2009). "Paeonol attenuates neurotoxicity and ameliorates cognitive impairment induced by d-galactose in ICR mice." Journal of the Neurological Sciences 277(1–2): 58-64. |
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[7]
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Peng, L.-H., et al. "Inhibitory effects of salidroside and paeonol on tyrosinase activity and melanin synthesis in mouse B16F10 melanoma cells and ultraviolet B-induced pigmentation in guinea pig skin." Phytomedicine (0). |
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[8]
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Sun, G.-P., et al. (2008). "Anti-tumor effects of paeonol in a HepA-hepatoma bearing mouse model via induction of tumor cell apoptosis and stimulation of IL-2 and TNF-α production." European Journal of Pharmacology 584(2–3): 246-252. |
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[9]
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Pan, L. L. and M. Dai (2009). "Paeonol from Paeonia suffruticosa prevents TNF-α-induced monocytic cell adhesion to rat aortic endothelial cells by suppression of VCAM-1 expression." Phytomedicine 16(11): 1027-1032. |
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[10]
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Li, X., et al. (2011). "Determination of trace paeonol by excited fluorescence scanning." Zhongchengyao 33(1): 179-182. |
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| 连结 |
 中药材图像数据库
药用植物图像数据库
中药标本数据库
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