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植物来源 |
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生物活性 |
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鉴定 |
熔点 |
223-224°C |
1HNMR
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| 分析方法 |
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| 仪器 |
5 × 10 cm TLC硅胶60 F254 板 (Merck, Darmstadt, Germany) |
| 流动相 |
乙醚: 乙酸乙酯 = 60: 40, v/v |
| 检测器 |
UV λ245 nm |
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| 仪器 |
Jasco (Tokyo, Japan) PU-1580 色谱泵, Jasco MD-910 光电二极管数组 (PDA) 检测器及一个 Varian Mod |
| 色谱柱 |
Phenomenex (Torrance, CA, USA) Gemini C18 反相色谱柱 (250 × 4.6 mm I.D., 5 µm) 配备一个Phenomenex C18 保护柱 (3 × 4.6 mm I.D., 5 µm) |
| 流动相 |
水: 甲醇 = 30: 70, v/v |
| 检测器 |
UV λ254 nm |
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| 仪器 |
Model 717 WISP 自动进样器, 一个model 510 泵, 一个荧光检测器model 474及一个Waters Associates (Milford, MA, USA) Millenium 32 Software Chromatography Manager 采集系统 |
| 色谱柱 |
Symmetry Shield RP18 (3.5 µm x 4.6mm x 150mm) HPLC 色谱柱, 相同质地的保护柱 (5µm x 4.6 mm x 20mm) |
| 流动相 |
甲醇: 水: 乙酸 = 65: 35: 0.2 |
| 检测器 |
λex = 410 nm 和 λem = 510 nm. |
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| 样品制备 |
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方法一 |
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于 Beijing Institute of New Technology Application, Beijing, China 装备的 Model GS 20 分析高速逆流色谱. 分析: 1.0 ml loop. 制备: 20 ml loop. |
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分析: 二乙醚-标准水; 固定相: 上有机相; 流动相: 35 ml 4.0% NaHCO3 和 55 ml 0.7% Na2CO3 和 80 ml 0.2% NaOH. 制备: 二乙醚-碱溶液系统. 固定相: 上有机相; 流动相: 120 ml 4.0% NaHCO3 和 240 ml 0.7% Na2CO3 和 480 ml 0.2% NaOH. |
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分析: 1.0 mL/min, 1500 rpm. 制备: 2.0 mL/min, 800 rpm |
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UV λ254 nm |
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| 参考文献 |
|
[1]
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Lee, H.-Z., et al. (2006). "Aloe-emodin induced DNA damage through generation of reactive oxygen species in human lung carcinoma cells." Cancer Letters 239(1): 55-63. |
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[2]
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Mandrioli, R., et al. (2011). "Determination of aloe emodin in Aloe vera extracts and commercial formulations by HPLC with tandem UV absorption and fluorescence detection." Food Chemistry 126(1): 387-393. |
|
[3]
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Chen, S.-H., et al. (2007). "Aloe-emodin-induced apoptosis in human gastric carcinoma cells." Food and Chemical Toxicology 45(11): 2296-2303. |
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[4]
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Lee, H.-Z., et al. (2001). "Effects and mechanisms of aloe-emodin on cell death in human lung squamous cell carcinoma." European Journal of Pharmacology 431(3): 287-295. |
|
[5]
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Lin, J.-G., et al. (2006). "Aloe-Emodin Induces Apoptosis in T24 Human Bladder Cancer Cells Through the p53 Dependent Apoptotic Pathway." The Journal of Urology 175(1): 343-347. |
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[6]
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Daud, M. M., et al. (2011). "P121 The anti proliferative effect of Aloin and Aloe emodin on MCF-7 and MDA-MB-231 breast cancer cells." The Breast 20, Supplement 1(0): S22. |
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[7]
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Tian, B. and Y. Hua (2005). "Concentration-dependence of prooxidant and antioxidant effects of aloin and aloe-emodin on DNA." Food Chemistry 91(3): 413-418. |
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[8]
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Lin, C.-W., et al. (2008). "Aloe-emodin is an interferon-inducing agent with antiviral activity against Japanese encephalitis virus and enterovirus 71." International Journal of Antimicrobial Agents 32(4): 355-359. |
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[9]
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Zaffaroni, M., et al. (2003). "High-performance liquid chromatographic assay for the determination of Aloe Emodin in mouse plasma." Journal of Chromatography B 796(1): 113-119. |
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[10]
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Yang, F., et al. (1999). "Preparative isolation and purification of hydroxyanthraquinones from Rheum officinale Baill by high-speed counter-current chromatography using pH-modulated stepwise elution." Journal of Chromatography A 858(1): 103-107. |
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| 连结 |
 中药材图像数据库
药用植物图像数据库
中药标本数据库
|
