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Natural Resources |
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Bioactivities |
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Identification |
Melting point |
216-220°C |
| Optical rotation |
[α]20D-34° (C=4.0g/100ml, water) |
1HNMR
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| Analytical Method |
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| INSTRUMENT |
Silica gel G plate |
| MOBILE PHASE |
CHCl3: Methanol: Ammonia Solution = 40: 7: 1 |
| DETECTION |
Ninhydrin Ninhydrin (DE) spray and heat at 105°C |
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| INSTRUMENT |
Waters Isocratic LC pump 510, with an automatic sample injection system (Waters 717 plus Autosampler) |
| COLUMN |
Bondopak C18 reverse phase column |
| MOBILE PHASE |
Methanol: pH 3 water with 10% v/v o-phosphoric acid = 40: 60, v/v, 1.2 mL/min |
| DETECTION |
UV λ217 nm |
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| INSTRUMENT |
Nicolet Nexus Model 870 FTIR equipped with a diffuse reflectance accessory (Nicolet model 0031-999) |
| COLUMN |
A: 100% aqueous buffer (2.5 mM NH4Ac, pH 7.4), B: 100% acetonitrile |
| MOBILE PHASE |
KBr FTIR grade = Wilmad |
| DETECTION |
Deuterated triglycine sulfate detector, all spectral measurements consisted of 128 coadded scans at 4 cm−1 resolution. |
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| INSTRUMENT |
HP3D capillary electrophoresis system (Hewlett Packard, Wilmington, DE, USA) |
| COLUMN |
Separation: Unmodified fused silica capillaries (80.5 cm × 75 μm internal diameter, effective length 72.0 cm) |
| MOBILE PHASE |
The cartridge temperature was maintained at 25°C, and injections were performed by pressure (2.5 kPa, 5 s). Applied voltage: range 15-30 kV. At the beginning of each day, the capillary was conditioned with 0.03 mol/L H3PO4 (10 min), water (2 min), and run buffer (15 min). Between injections, the capillary was purged with 0.03 mol/L H3PO4 (1 min), water (1 min), and run buffer (2 min). The applied pressure for rinsing the capillary was 90 kPa. |
| DETECTION |
UV λ210 nm |
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| INSTRUMENT |
Two LKB 2150 HPLC pumps (LKB Bromma, Sweden) controlled by Gradient Controller LKB LC 2152 and connected by Rheodyne high pressure gradient mixer (Rheodyne, Redwood, CA) and were equipped with a Rheodyne 7125 manual injector with a 10-μl sample loop. |
| COLUMN |
Nucleosil® C18 pre-packed HPLC column |
| MOBILE PHASE |
A: Mixture of acetonitrile and water (5: 95 v/v), pH 2.40±0.1 adjusted with o-phosphoric acid, B: Mixture of acetonitrile and water (40: 60 v/v), pH 2.40±0.1 adjusted with o-phosphoric acid, 0-5.1 min 2% B, 5.1-10 min 2-100% B, 10-15 min 100% B, 1.50 mL/min |
| DETECTION |
BC LC 1210 detector, 220 nm at start time, to 240 nm at 3.70 min and again to 220 nm at 10.70 min |
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| Reference |
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[1]
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Eng, A. T. W., et al. (2007). "Evaluation of surfactant assisted pressurized liquid extraction for the determination of glycyrrhizin and ephedrine in medicinal plants." Analytica Chimica Acta 583(2): 289-295. |
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[2]
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Featherstone, N., et al. "Ephedrine hydrochloride: Novel use in the management of resistant non-neurogenic daytime urinary incontinence in children." Journal of Pediatric Urology (0). |
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[3]
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Wu, G. and Y. Zhang (2009). "Quality standard of Zhiqiguanyan tablets." Zhongguo Yaopin Biaozhun 10(3): 186-189. |
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[4]
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Şentürk, Z., et al. (2002). "Determination of theophylline and ephedrine HCL in tablets by ratio-spectra derivative spectrophotometry and LC." Journal of Pharmaceutical and Biomedical Analysis 29(1–2): 291-298. |
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[5]
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Dijiba, Y. K., et al. (2005). "Determinations of ephedrine in mixtures of ephedrine and pseudoephedrine using diffuse reflectance infrared spectroscopy." International Journal of Pharmaceutics 289(1–2): 39-49. |
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[6]
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Zheng, Y., et al. (2013). "Ephedrine hydrochloride inhibits PGN-induced inflammatory responses by promoting IL-10 production and decreasing proinflammatory cytokine secretion via the PI3K/Akt/GSK3β pathway." Cell. Mol. Immunol. 10(4): 330-337. |
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[7]
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Phinney, K. W., et al. (2005). "Determination of ephedrine alkaloid stereoisomers in dietary supplements by capillary electrophoresis." Journal of Chromatography A 1077(1): 90-97. |
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[8]
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Boberić-Borojević, D., et al. (1999). "Simultaneous assay of ephedrine hydrochloride, theophylline, papaverine hydrochloride and hydroxyzine hydrochloride in tablets using RP-LC." Journal of Pharmaceutical and Biomedical Analysis 21(1): 15-22. |
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| Link to |
 Chinese Medicinal Material Images Database
Medicinal Plant Images Database
Chinese Medicine Specimen Database
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