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植物来源 |
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生物活性 |
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|
鉴定 |
熔点 |
185-188°C |
| 旋亮度 |
[α]20D+62° (C=1.0g/100ml, 水) |
1HNMR
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| 分析方法 |
|
| 仪器 |
硅胶 60 F254 |
| 流动相 |
正己烷: 丙酮: 乙酸乙酯: 三乙胺: 水 = 3: 4: 3: 0.1: 0.3, v/v/v/v/v. |
| 检测器 |
UV λ208 nm |
|
|
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| 仪器 |
Waters® 组合系统 (Milford, Massachusetts, USA) |
| 色谱柱 |
Waters® C18 Nova-Pak® 色谱柱 (4 μm, 3.9 mm × 150 mm) |
| 流动相 |
0.12 M 三水合乙腈钠乙腈缓冲溶液, 浓乙酸调节pH值至4.0: 乙腈: 甲醇 = 45: 47: 8, 0.8 mL/min |
| 检测器 |
UV λ214 nm |
|
|
|
| 仪器 |
CHEMITO 5210 气相色谱包括一个 Oracle 2 integrator和一个火焰离子化检测器。 |
| 色谱柱 |
10% OV 1 Chromosorb W-HP (80-100 Mesh) 担体填充 S.S. 色谱柱 (长2.50 m直径2 mm), Sky Lab, Thane, Mumbai 制造. |
| 流动相 |
氮气, 柱流速: 30 mL/min, 柱箱起始温度: 135°C 1分钟, 每分钟上升 10°C 至 250°C 保持 5 分钟, 进样器和检测器接口温度: 280°C |
| 检测器 |
火焰离子化检测器 |
|
|
|
| 仪器 |
Beckman 毛细管电泳仪 P/ACE 5510 (Beckman Instrument, Fullerton, USA) 包括一个二极管数组检测系统, 自动进样器, 一个流体冷却塔筒及 System Gold Data 工作站 |
| 色谱柱 |
分离: 75 μm View the MathML source (50 cm to window) 的熔融石英毛细管柱 (Beckman) |
| 流动相 |
20 mmol/l pH 7.0 PBS |
| 检测器 |
UV λ200 nm |
|
|
|
| 仪器 |
Shimadzu Corporation 集成高校液相色谱系统 LC-2010A (Chromatographic and Spectrophotometric Division, Kyoto, Japan) |
| 色谱柱 |
Supelcosil™ LC-Si 色谱柱, 25 cm × 4.6 mm, 包括5 μm 硅胶粒 (Supelco, Switzerland; Part No. 58295) |
| 流动相 |
甲醇: 水 (每升6.0 g 醋酸铵及10 mL 三乙胺, 磷酸调节 pH 值至 5.2) = 95: 5 (v/v), 1.2 mL/min |
| 检测器 |
UV λ254 nm |
|
| 样品制备 |
|
方法一 |
|
|
|
|
|
HPE-100 CE 系统12 kV 最大电压 (BioRad, Hercules, CA, USA) |
|
|
依次用未涂层石英毛细管冲洗蒸馏水(5 分钟), 0.1 M NaOH (5 分钟), 再冲洗蒸馏水 (5 分钟), 通过循环水真空泵用缓冲溶液冲洗毛细管出口用储器 (5 分钟) |
|
|
柱塞泵发动载流, 蠕动泵将样品溶液分别加载 16 项喷射阀的一个样品环及两个试剂环 (容积: 分别 20 μl) |
|
|
UV λ214 nm |
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| 参考文献 |
|
[1]
|
Abdelwahab, N. S. and E. A. Abdelaleem (2013). "TLC-densitometric determination of guaifenesin, pseudoephedrine hydrochloride and guaifenesin related substance (guaiacol)." J. Planar Chromatogr.--Mod. TLC 26(1): 73-77. |
|
[2]
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Eng, A. T. W., et al. (2007). "Evaluation of surfactant assisted pressurized liquid extraction for the determination of glycyrrhizin and ephedrine in medicinal plants." Analytica Chimica Acta 583(2): 289-295. |
|
[3]
|
Gu, X., et al. (2005). "Simultaneous analysis of the H1-antihistamine acrivastine and the decongestant pseudoephedrine hydrochloride by high-performance liquid chromatography." Journal of Pharmaceutical and Biomedical Analysis 37(4): 663-667. |
|
[4]
|
Raj, S. V., et al. (1998). "Simultaneous determination of pseudoephedrine hydrochloride and diphenhydramine hydrochloride in cough syrup by gas chromatography (GC)." Talanta 46(1): 221-225. |
|
[5]
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Zhang, L., et al. (2000). "Simultaneous determination of the active ingredients in composite pseudoephedrine hydrochloride tablets by capillary electrophoresis." Analytica Chimica Acta 424(2): 257-262. |
|
[6]
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Ali, M. S., et al. (2007). "A new hydrophilic interaction liquid chromatographic (HILIC) procedure for the simultaneous determination of pseudoephedrine hydrochloride (PSH), diphenhydramine hydrochloride (DPH) and dextromethorphan hydrobromide (DXH) in cough-cold formulations." Journal of Pharmaceutical and Biomedical Analysis 43(1): 158-167. |
|
[7]
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Liu, X., et al. (2007). "Separation and determination of four active components in medicinal preparations by flow injection-capillary electrophoresis." Journal of Pharmaceutical and Biomedical Analysis 43(5): 1700-1705. |
|
[8]
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Kiyoshi Minamizawa, et al. (2006) "Effect of d-Pseudoephedrine on Cough Reflex and Its Mode of Action in Guinea Pigs." Journal of Pharmacological Sciences 102(1): 136–142. |
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| 连结 |
 中药材图像数据库
药用植物图像数据库
中药标本数据库
|
