化学名称 α-细辛脑
外观 针晶
化学物质登录号 2883-98-9
分子式
分子量 208.25
植物来源
生物活性
鉴定 熔点 62-63°C
UV
IR
ESI-MS
1HNMR
13CNMR
分析方法
仪器 硅胶板GF254 10 × 10 cm (Merck, Darmstadt, Germany)
流动相 甲苯: 乙酸乙酯 = 8: 2
检测器 UV λ365 nm
仪器 LC10 HPLC仪
色谱柱 Krouasil C18 (4.6mm x 250 mm, 5μm) 色谱柱, 25°C
流动相 甲醇: 磷酸二氢钾 = 55: 45, 0.8 mL/min
检测器 UV λ331 nm
仪器 HP-5980 series II GC仪
色谱柱 HP-WAX 和 HP-5 毛细管色谱柱 (30 m × 0.25 mm i.d. × 0.25 µm 膜)
流动相 程序: 60-300°C, 10°C/min, 进样温度: 270°C, 检测器温度: 300°C, 载气: 氮气 = 2 mL/min, 分离比例: 20: 1, 进样量: 0.5 µl
检测器 双FID检测器
仪器 HP 6890 GC 系统
色谱柱 硅胶熔融毛细管色谱柱 HP-5MS (30 m x 0.25 mm i.d. 0.25 m 膜) Agilent, USA
流动相 载气: 氦气1.0 mL/min. 不分离 (2.0 min) 与分离模式. 进样器: 270°C, 色谱柱: 60-160°C, 10°C/min, 160-300°C, 12°C/min, 保持 5 min, 质谱: 230°C.
检测器 HP MD5973四极杆质谱
样品制备
方法一
Model TBE-300A 仪器 (Shanghai, Tauto Biotech, China) 包括三个PTFE制备线圈 (内径, 1.6 mm;总容量, 260 mL)及一个20-mL样品回路, model NS-1007 恒流泵 (Beijing Emilion Science & Technology, Beijing, China), model 8823AUV 检测器 (Beijing Emilion Science & Technology).
正己烷: 乙酸乙酯: 甲醇: 水 = 1: 0.2: 1: 0.3, 流动相: 下相
1.5 mL/min, 800 rpm
UV λ254 nm
参考文献
[1] Wang, D., et al. (2011). "An efficient combination of supercritical fluid extraction and high-speed counter-current chromatography to extract and purify (E)- and (Z)-diastereomers of α-asarone and β-asarone from Acorus tatarinowii Schott." J. Sep. Sci. 34(23): 3339-3343.
[2] Tscurevitch, N. N., et al. (2012). "Analysis of composition of essential oil and standardization of homeopathic matrix tinctures of Asarum europaeum." Vopr. Biol., Med. Farm. Khim. (3): 16-21.
[3] Sandeep, D. and C. K. K. Nair (2011). "Radioprotection by α-asarone: Prevention of genotoxicity and hematopoietic injury in mammalian organism." Mutat. Res., Genet. Toxicol. Environ. Mutagen. 722(1): 62-68.
[4] Yang, L., et al. (2008). "Effects of Acorus gramineus and its component, alpha-asarone, on apoptosis of hippocampal neurons after seizure in immature rats." Neural Regener. Res. 3(1): 19-24.
[5] Yang, L.-b., et al. (2006). "Effect of acorus gramineus and its active component α-asarone on apoptosis of hippocampal neurons in epileptic young rats." Zhongcaoyao 37(8): 1196-1199.
[6] Marczewska, J., et al. (2013). "Assessment of the genotoxic activity of α-asarone and its derivatives in the comet assay." Acta Pol. Pharm. 70(2): 349-354.
[7] Garduno, L., et al. (1997). "Hypolipidemic activity of α-asarone in mice." J. Ethnopharmacol. 55(2): 161-163.
[8] Manikandan, S. and R. S. Devi (2005). "Antioxidant property of α-asarone against noise-stress-induced changes in different regions of rat brain." Pharmacol. Res. 52(6): 467-474.
[9] Wu, Y., et al. (2011). "Effect of α-asarone on construction of intractable epilepsy cell models by culturing hippocampal neurons with magnesium-free extracellular solution." Zhongguo Zuzhi Gongcheng Yanjiu Yu Linchuang Kangfu 15(33): 6139-6142.
[10] Dong, W., et al. (2010). Extraction method of effective compounds from Chinese medicine Acorus and its application as anti-Alzheimers agents, Shanghai Institute of Pharmaceutical Industry, Peop. Rep. China. 13pp.
[11] Han, L., et al. (2009). "Research on anticonvulsive and antiepileptic effect of α-asarone and its mechanism." Zhongguo Yaolixue Tongbao 25(7): 963-966.
[12] Gu, Q., et al. (2010). "Effects of α-asarone on the glutamate transporter EAAC1 in Xenopus oocytes." Planta Med. 76(6): 595-598.
[13] Fan, Y., et al. (1999). "The effect of α-asarone on trachea cilia movement." Guangxi Yike Daxue Xuebao 16(2): 173-174.
[14] Oprean, R., et al. (1998). "Comparison of GC-MS and TLC techniques for asarone isomers determination." Journal of Pharmaceutical and Biomedical Analysis 18(1–2): 227-234.
[15] Zhang, H., et al. (2009). "Determination of α-asarone in different Radix et Rhizoma Asari by HPLC." Yiyao Daobao 28(10): 1349-1351.
[16] Deng, C., et al. (2004). "Rapid determination of essential oil in Acorus tatarinowii Schott. by pressurized hot water extraction followed by solid-phase microextraction and gas chromatography-mass spectrometry." J Chromatogr A 1059(1-2): 149-155.
连结 中药材图像数据库 药用植物图像数据库 中药标本数据库

本记录之永久网址: https://sys01.lib.hkbu.edu.hk/cmed/pid/detail.php?code=P00087