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植物来源 |
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生物活性 |
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鉴定 |
熔点 |
114-116°C |
| 旋亮度 |
[α]-0.65°至-0.75° |
1HNMR
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| 分析方法 |
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| 仪器 |
硅胶60F254 板, 厚度0.25 mm, Merck (Darmstadt, Germany) |
| 流动相 |
甲醇: 乙酸乙酯 = 40: 60 % v/v |
| 检测器 |
德里根道尔夫试剂, UV λ254 nm |
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| 仪器 |
液相色谱仪 (Prominence Liquid Chromatograph LC-20 AD, Shimadzu) |
| 色谱柱 |
Ascentis Express C18 色谱柱 (50 mm × 2.1 mm, 2.7 μm, Supelco, USA) |
| 流动相 |
A: 1% v/v 甲酸水, B: 1% v/v 甲酸甲醇, 0 min 10% B, 10 min 90% B, 17 min 90% B, 17.1 min 10% B, 23 min 10% B, 0.2 mL/min |
| 检测器 |
MS 检测器电喷雾离子源和四极矩分析仪 (Liquid Chromatograph Mass Spectrometer LCMS-2020, Shimadzu), 检测电压: 0.95 kV |
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| 仪器 |
JASCO Model 880-PU 泵 (Japan Spectroscopic, Tokyo, Japan), Rheodyne 注射进样器, Model 7125, 一个10-μl 回路 (Rheodyne, Berkeley, CA, USA), JASCO Model 860-CO 柱温箱, JASCO Model 821-FP荧光检测器 |
| 色谱柱 |
Cosmocil 5C18-MS 柱 (Nacalai Tesque, Tokyo, Japan) (150 × 4.6 mm I.D.; 5 μm) |
| 流动相 |
乙腈: 0.02 M 十二烷基硫酸钠 = pH 3.5 的硫酸 = 60: 40, v/v, 1.0 mL/min |
| 检测器 |
JASCO Model 821-FP 荧光检测器激发及吸收波长为 255 和 474 nm |
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| 仪器 |
MPI-A 电泳仪 (Xi’an Remex Electronic Science Tech Co., Ltd., Xi’an, China) 配备高压电源, EC 恒电释器, 发光检测器, 及资料分析器. |
| 色谱柱 |
长18 cm 未涂层熔石英毛细管 (25 μm i.d., 360 μm o.d.) |
| 流动相 |
双蒸馏水, 纯乙腈, 电泳缓冲液冲洗: 2.5 mM TBAP, 1M HAc, 20 mM NaAc 中混合乙腈和丙二醇, 10 min |
| 检测器 |
CH Instrument model 800 伏安分析仪 (Austin, TX, USA) |
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| 样品制备 |
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方法一 |
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TBE-300 HSCCC 仪 (Tauto Biotechnique, Shanghai, China), 配备三个串联多层线圈分离柱 (id 51.5 mm, 总体积 5300 mL), 20 mL 进样回路 |
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乙酸乙酯: 正丁醇: 水 = 4: 1: 5, v/v. 上层有机相加入0.50% TFA, 下层水相加入0.15% HCl |
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1.0 mL/min, 850 rpm |
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Agilent 1200 HPLC 系统分析及 Agilent HPLC 工作站 (Agilent, USA) |
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| 参考文献 |
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[1]
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Zhang, X.-q., et al. (2012). "Efficacy of non-continuous injection and continuous pumping of atropine in treatment of organophosphorous poisoning." Chuanbei Yixueyuan Xuebao 27(4): 414-416. |
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[2]
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Liu, Z.-j., et al. (2012). "Comparison of effect of penehyclidine hydrochloride and atropine on gastrointestinal hormone in mice." Linchuang Mazuixue Zazhi 28(7): 703-704. |
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[3]
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Dar, R. A., et al. (2012). "Electrochemical determination of atropine at multi-wall carbon nanotube electrode based on the enhancement effect of sodium dodecyl benzene sulfonate." Colloids and Surfaces B: Biointerfaces 91(0): 10-17. |
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[4]
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Okuda, T., et al. (1991). "Determination of atropine in biological specimens by high-performance liquid chromatography." Journal of Chromatography B: Biomedical Sciences and Applications 567(1): 141-149. |
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[5]
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Lehr G.J., Yuen S.M., Lawrence G.D. "Liquid chromatographic determination of atropine in nerve gas antidotes and other dosage forms." (1995) Journal of AOAC International, 78(2), pp. 339-343. |
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[6]
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Polak, B. and A. Rompala (2007). "Effect of acidic mobile phase additives on the TLC behaviour of some alkaloids." Acta Chromatogr. 18: 24-35. |
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[7]
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Jakabová, S., et al. (2012). "Determination of tropane alkaloids atropine and scopolamine by liquid chromatography–mass spectrometry in plant organs of Datura species." Journal of Chromatography A 1232(0): 295-301. |
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[8]
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Takahashi, M., et al. (1997). "Determination of atropine in pharmaceutical preparations by liquid chromatography with fluorescence detection." Journal of Chromatography A 775(1–2): 137-141. |
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[9]
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Yuan, B., et al. (2010). "Simultaneous determination of atropine, anisodamine, and scopolamine in plant extract by nonaqueous capillary electrophoresis coupled with electrochemiluminescence and electrochemistry dual detection." Journal of Chromatography A 1217(1): 171-174. |
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[10]
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He, Y., et al. (2011). "Preparative separation of atropine and scopolamine from Daturae metelis Flos using pH-zone-refining counter-current chromatography with counter-rotation and dual-mode elution procedure." Journal of Separation Science 34(7): 806-811. |
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| 连结 |
 药用植物图像数据库
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