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植物来源 |
|
|
生物活性 |
|
|
鉴定 |
熔点 |
218°C |
|
|
| 分析方法 |
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| 仪器 |
硅胶60 TLC玻璃板 (Merck, Darmstad, Germany) |
| 流动相 |
氯仿/甲醇/氨 (60: 35: 8) |
|
|
|
| 仪器 |
Merck-Hitachi HPLC 仪配备 L-7200 自动进样器 |
| 色谱柱 |
反相C18 色谱柱 (25 × 0.46 cm Tracer Extrasil ODS-2, 5 μm; Teknokroma, Barcelona, Spain); 2 × 20 mm保护柱 (Upchurch Scientific, Oak Harbor, WA, USA) |
| 流动相 |
甲醇: 21% (v/v); 10 mM 庚烷磺酸作离子对; 0.3 M 磷酸钠缓冲液: pH 3; 1 mL/min |
| 检测器 |
UV λ225 nm |
|
|
|
| 仪器 |
Model 6890N GC 仪 (Agilent Technologies, DE, USA) |
| 色谱柱 |
HP-5 (Agilent Technologies, Palo Alto, CA, USA) 硅胶熔融毛细管柱 (30 m × 0.25 mm ID) 厚度0.33 μm |
| 流动相 |
氦气, 1.3 mL/min, 初始柱温 60°C, 10°C/min 速率提升至终温度 275°C. 进样器及辅助温度为 250°C 和 275°C |
| 检测器 |
5973 质量选择检测器 (Agilent Technologies, DE, USA), 全扫描电子冲击 (EI), 离子选择检测模式 (SIM), 离子化电压: 70 eV, 分辨率: 高, 品质范围: 35-350 amu, 保压时间: 100 ms. 四极管: 150°C. 电子倍增器电压 1200 V. 时间安排选择离子监测模式质谱 96 和128m/z |
|
| 样品制备 |
|
方法一 |
|
|
干燥虫样品 (6 kg); 研碎以 50% 乙醇溶液提取两次 (40 L). 热滤并浓缩. 乙酸乙酯提取水溶液. 浓缩, 过夜, 得白色沉淀. 硅胶色谱柱填充氯仿-甲醇以乙酸乙酯梯度纯化得无色针晶. |
|
|
| 参考文献 |
|
[1]
|
Karras, D. J., et al. (1996). "Poisoning from Spanish fly (cantharidin)." The American Journal of Emergency Medicine 14(5): 478-483. |
|
[2]
|
Efferth, T., et al. (2005). "Molecular modes of action of cantharidin in tumor cells." Biochemical Pharmacology 69(5): 811-818. |
|
[3]
|
Verma, A. K. and S. B. Prasad (2012). "Bioactive component, cantharidin from Mylabris cichorii and its antitumor activity against Ehrlich ascites carcinoma." Cell Biol. Toxicol. 28(3): 133-147. |
|
[4]
|
Wang, C.-C., et al. (2000). "Cytotoxic effects of cantharidin on the growth of normal and carcinoma cells." Toxicology 147(2): 77-87. |
|
[5]
|
Knapp, J., et al. (1998). "Contractility and Inhibition of Protein Phosphatases by Cantharidin." General Pharmacology: The Vascular System 31(5): 729-733. |
|
[6]
|
Romero, G., et al. (2004). "Tratamiento tópico con cantaridina de moluscos contagiosos." Actas Dermo-Sifiliográficas 95(9): 548-552. |
|
[7]
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Moulharat, N., et al. (2008). "Molecular pharmacology of adipocyte-secreted autotaxin." Chemico-Biological Interactions 172(2): 115-124. |
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[8]
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Moreno-Delgado, D., et al. (2007). "Phosphatases regulate histamine synthesis in rat brain." Neuroscience 150(3): 616-624. |
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[9]
|
Mehdinia, A., et al. (2011). "Analysis of cantharidin in false blister beetles (Coleoptera: Oedemeridae) by headspace solid-phase microextraction and gas chromatography-mass spectrometry." J. Chromatogr. B: Anal. Technol. Biomed. Life Sci. 879(27): 2897-2901. |
|
[10]
|
Wang, C.-C., et al. (2000). "Cytotoxic effects of cantharidin on the growth of normal and carcinoma cells." Toxicology 147(2): 77-87. |
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| 连结 |
 中药材图像数据库
中药标本数据库
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