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植物來源 |
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異名 |
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生物活性 |
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鑑定 |
熔點 |
259.5-260°C |
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| 分析方法 |
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| 儀器 |
Waters HPLC 儀包括1525 四元泵, 2996 UV-Vis光電二極管數組檢測器, 717 自動進樣器, Empower 工作站 |
| 色譜柱 |
Hypersil BDS C18 色譜柱 (200 mm × 4.6 mm, id 5 μm)及保護柱 (4.0 mm × 3.0 mm, id 5 μm) |
| 流動相 |
A: 水, B: CAN, 0–30 min, 40–50% B; 30–35 min, 50–75% B; 35–55 min, 75–80% B; 55–60 min, 80% B, 1.0 mL/min |
| 檢測器 |
UV λ225 nm |
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| 儀器 |
HPLC 系統 (Hitachi, Japan) 及 Hitachi 泵 (model L-2130), 紫外可見光檢測器 (Hitachi, model L-2400) |
| 色譜柱 |
Diamonsil C18 分離柱 (5 μm, 250 mm × 4.6 mm i.d., Dikma Technology, Beijing, China) |
| 流動相 |
乙腈: 水: 四氫呋喃: 乙酸 (51: 48: 1: 0.1, v/v/v/v), 1 mL/min |
| 檢測器 |
UV λ254 nm |
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| 儀器 |
Waters Quanta 4000 毛細管電泳系統及 UV 檢測器 |
| 色譜柱 |
70cm × 75µm I.D. 矽膠熔融毛細管柱 (Polymicro, Phoenix, AZ, USA); 進樣時間: 2s; 靜水力; 25kV; 24.5-25.0°C ; 時間: 15 min |
| 流動相 |
pH 7.31 40mM SDS 緩衝液, 20mM NaH2PO4 和9mM Na2B4O7-乙腈 (3: 2) |
| 檢測器 |
UV λ254 nm |
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| 樣品製備 |
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方法一 |
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FZ102 打粉機打粉. 800 ml 乙酸乙酯 30 min, 超聲提取 45 min. 重複兩次, 合併. 減壓蒸幹. |
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TBE-300A HSCCC (Tauto Biotechnique Company, Shanghai, China) 包括三多重線圈分離柱 (I.D. = 1.6 mm, 總量 = 260 ml) 和 20 ml 樣品回路 |
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上相 (固定相) 及下相 (流動相), 正己烷-乙酸乙酯-甲醇-水 (5: 5: 7: 5, v/v) |
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2.0 mL/min; 900 rpm |
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UV λ254 nm |
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| 參考文獻 |
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[1]
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Kim, S.-J., et al. (2009). "Rutecarpine ameliorates bodyweight gain through the inhibition of orexigenic neuropeptides NPY and AgRP in mice." Biochemical and Biophysical Research Communications 389(3): 437-442. |
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[2]
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de Barros Corrêa, D., et al. (1975). "Dihydrocinnamic acids from Hortia badinii." Phytochemistry 14(9): 2059-2060. |
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[3]
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Ji, S. and P. Qi (2012). "Antihypertensive mechanism of rutaecarpine in hypertension rats." Zhongguo Shenghua Yaowu Zazhi 33(3): 237-240. |
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[4]
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Jia, S. and C. Hu (2010). "Pharmacological effects of rutaecarpine as a cardiovascular protective agent." Molecules 15: 1873-1881. |
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[5]
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Wang, C.-L., et al. (2006). "Progress in the synthesis of rutaecarpine." Youji Huaxue 26(10): 1437-1443. |
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[6]
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Zhang, P. T., et al. (2013). "Simultaneous Quantification of Limonin, Two Indolequinazoline Alkaloids, and Four Quinolone Alkaloids in Evodia rutaecarpa (Juss.) Benth by HPLC-DAD Method." J Anal Methods Chem 2013: 827361. |
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[7]
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Yang, J.-w., et al. (2012). "Protective effect of rutaecarpine on acute myocardial ischemia in animals." Zhongguo Shenghua Yaowu Zazhi 33(6): 776-779. |
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[8]
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Zhu, Q.-N., et al. (2013). "Rutaecarpine effects on expression of hepatic phase-1, phase-2 metabolism and transporter genes as a basis of herb-drug interactions." J. Ethnopharmacol. 147(1): 215-219. |
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[9]
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Liu, R., et al. (2005). "Preparative isolation and purification of alkaloids from the Chinese medicinal herb Evodia rutaecarpa (Juss.) Benth by high-speed counter-current chromatography." Journal of Chromatography A 1074(1–2): 139-144. |
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[10]
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Lee, M.-C., et al. (1996). "Determination of the alkaloids in Evodiae fructus by capillary electrophoresis." Journal of Chromatography A 755(1): 113-119. |
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| 連結 |
 中藥材圖像數據庫
藥用植物圖像數據庫
中藥標本數據庫
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