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植物來源 |
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生物活性 |
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|
鑑定 |
熔點 |
232-233°C |
| 旋光度 |
[α]D-56° (氯仿) |
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|
| 分析方法 |
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| 儀器 |
TLC 板 (20 × 10 cm, 0.25 mm) 矽膠F254, Merck |
| 流動相 |
氯仿: 甲醇: 25% 氨水 = 50: 0.5: 0.1, v/v/v |
| 檢測器 |
UV λ220 nm |
|
|
|
| 儀器 |
Agilent 1100 HPLC 儀 (Waldbronn, Germany) |
| 色譜柱 |
Waters Xterra C18 色譜柱 (5 µm, 3.9 mm × 150 mm, USA) |
| 流動相 |
A: 0.1% 甲酸20 mM 醋酸銨, B: 0.1% 甲酸乙腈. 0 min 10% B, 0-20 min 梯度提高至 40% B, 20-30 min 10% B; 0.7 mL/min; 柱溫: 40°C |
| 檢測器 |
UV λ220 nm |
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|
|
| 儀器 |
Waters Acquity UPLC™ 系統 (Waters Corp., Milford, MA, USA) |
| 色譜柱 |
Acquity UPLC™ HSS T3 色譜柱 (100 mm × 2.1 mm I.D., 1.8 µm) 配備 LC-18 保護柱 (Vanguard 2.1 mm × 5 mm, Waters Corp., Milford, MA, USA) |
| 流動相 |
A: 50 mM 甲酸銨, B: 乙腈 (0.05% 甲酸), 0 min 85% A 或 0-5 min 15% B 等度, 0-3 min 50% A 或 5-8 min 50% B, 3-10 min 30% A 或 8-15 min 70% B, 15-18 min 100% B, 分離重平衡後 100% B 沖洗3.5 min; 0.25 mL/min |
| 檢測器 |
UV λ220 nm |
|
|
|
| 儀器 |
Agilent Series 1100 HPLC 儀 |
| 色譜柱 |
保護柱 (Supelco, Bellefonte, PA, USA) |
| 流動相 |
A: 0.1% 甲酸水, B: 0.1% 甲酸乙腈, 0 min 87% A 或0-15 min 13% B, 0-2 min 50% A 或15-17 min 50% B, 2-18 min 100% B 或17-25 min 100% B; 分離重平衡後100% B 沖洗13 min, 87% A 或 13% B; 0.5 mL/min |
| 檢測器 |
HPLC-ESI-TOF-MS 色譜-質譜儀 (Model #G1969A, Agilent Technologies, Palo Alto, CA, USA), 包括 ESI 源; 毛細管電壓: 3500 V; 氮氣: 11 L/min, 350°C ; 光電倍增管 (PMT): 850V, 100V 和 60V; 全掃描: m/z 100-900 |
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| 儀器 |
Waters Acquity UPLC BEH C18 色譜柱 (2.1 mm × 100 mm, 1.7 µm) 和 Micromass ZQ 系統 |
| 色譜柱 |
LC-18保護柱 (Vanguard 2.1 mm × 5 mm Waters Corp., Milford, MA, USA) |
| 流動相 |
A: 乙腈 A, B: 10 mM 甲酸銨用 0.1% (v/v) 25% 氨溶液調節, 0-5 min 5-20% A, 5-7 min 20-40% A, 7-8.5 min 40-90% A |
| 檢測器 |
LCQ 離子阱質譜 (ThermoFinnigan, San Jose, CA, USA), 正離子模式, 毛細管溫度: 300°C, 源電壓: 5 kV, 毛細管電壓: 9 V, 品質範圍: m/z 100-800, 碰撞氣, 氦氣, 相對碰撞能: 35-40% |
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| 參考文獻 |
|
[1]
|
Jiang, Z., et al. (2009). "Determination of senkirkine and senecionine in Tussilago farfara using EXTRACTION and pressurized hot water extraction with liquid chromatography tandem mass spectrometry." Talanta 79(2): 539-546. |
|
[2]
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Avula, B., et al. (2012). "Simultaneous determination of sesquiterpenes and pyrrolizidine alkaloids from the rhizomes of Petasites hybridus (L.) G.M. et Sch. and dietary supplements using UPLC-UV and HPLC-MS methods." Journal of Pharmaceutical and Biomedical Analysis 70(0): 53-63. |
|
[3]
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Yang, X., et al. (2011). "Authentication of Senecio scandens and S. vulgaris based on the comprehensive secondary metabolic patterns gained by UPLC-MS." Journal of Pharmaceutical and Biomedical Analysis 56(2): 165-172. |
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[4]
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Chen, Y., et al. (2009). "Intracellular glutathione plays important roles in pyrrolizidine alkaloids-induced growth inhibition on hepatocytes." Environmental Toxicology and Pharmacology 28(3): 357-362. |
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[5]
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Han, J. and A. Liang (2011). "Effect of senecionine on in vitro cultured mouse embryo development by whole embryo culture method." Shengtai Duli Xuebao 6(2): 189-194. |
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[6]
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Mroczek, T., et al. (2002). "Simultaneous determination of N-oxides and free bases of pyrrolizidine alkaloids by cation-exchange solid-phase extraction and ion-pair high-performance liquid chromatography." Journal of Chromatography A 949(1–2): 249-262. |
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| 連結 |
 中藥材圖像數據庫
藥用植物圖像數據庫
中藥標本數據庫
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