化学名称 腺苷
外观 白色粉末
化学物质登录号 58-61-7
分子式
分子量 267.24
植物来源
生物活性
鉴定 熔点 234-236°C
旋亮度 [α]27D-5° (C=0.25g/100ml, 水)
1HNMR
分析方法
色谱柱 铝板 (10 cm × 10 cm) 预制硅胶60 F254 (0.2 mm) (Merck, Darmstadt, Germany)
流动相 A: 正丁醇, 水, 乙腈, 10% 氨溶液, 冰醋酸 (5: 2: 4: 1: 0.5, v/v)
B: 正丁醇/氯仿/已经/10% 氨溶液/冰醋酸 (5: 4: 2: 1: 0.5, v/v)
检测器 UV λ254 nm
仪器 Hewlett-Packard (HP) 1050 Series HPLC仪配置一个可变波长 UV 吸收检测器 (VWD, part no. 79853C)
色谱柱 Agilent Zorbax Eclipse XDB-C18 色谱柱 (150 × 4.6 mm)
流动相 乙腈: 去离子水 = 7: 93, 1.0 mL/min
检测器 UV λ260 nm
仪器 Perkin-Elmer Model Series 4 色谱仪 (Norwalk, PA), 一个Rheodyne 7125 (Berkeley, CA) 注射阀及一个20-μl回路, model Perkin-Elmer LC235 二极管数组UV 检测器.
色谱柱 250 × 4.6 mm i.d. 不锈钢 Hibar 色谱柱预装一个 5 μm RP-18相 (Hypesil, Runcorn, UK); 色谱柱用一个 LiChroCart 4-4 保护柱系统 (Merck) 保护.
流动相 乙腈: 0.01 M 磷酸二氢钠 = 5: 95, 1.0 mL/min
检测器 UV λ260 nm
仪器 LC 系统 (Waters 2690 Separation Module, USA) 配置一个可变波长检测器 (Waters™ 486, Tunable Absorbency Detector) 及一个自动进样器 (Waters, USA).
色谱柱 Hypersil® BDS, C18 色谱柱, 5 μ, 150 × 4.6 mm.
流动相 A: 甲醇, B: 1% 醋酸溶液, 4: 1, 1.5 mL/min
检测器 UV λ265 nm
样品制备
方法一
TBE-300A 高速逆流色谱 (Tauto biotech., Shanghai, China) 配置三个多层线圈分离柱与系统连接 (I.D. 管 = 1.6 mm, 总体积 = 260 ml) 和一个 20 ml 进样回路.
乙酸乙酯: 正丁醇: 水 = 1: 4: 5, v/v/v
1.5 mL/min
UV λ254 nm
参考文献
[1] Xue, X. F., Zhou, J. H., Wu, L. M., Fu, L. H., & Zhao, J. (2009). "HPLC determination of adenosine in royal jelly." Food Chemistry, 115(2), 715-719. doi: http://dx.doi.org/10.1016/j.foodchem.2008.12.003
[2] Ling, J. Y., Zhang, G. Y., Lin, J. Q., Cui, Z. J., & Zhang, C. K. (2009). "Supercritical fluid extraction of cordycepin and adenosine from Cordyceps kyushuensis and purification by high-speed counter-current chromatography." Separation and Purification Technology, 66(3), 625-629. doi: http://dx.doi.org/10.1016/j.seppur.2008.12.022
[3] Konrad, F. M., Neudeck, G., Vollmer, I., Ngamsri, K. C., Thiel, M., & Reutershan, J. (2013). "Protective effects of pentoxifylline in pulmonary inflammation are adenosine receptor A2A dependent." FASEB J., 27(9), 3524-3535, 3510.1096/fj.3513-228122. doi: 10.1096/fj.13-228122
[4] Hasko, G., et al. (2000). "Adenosine inhibits IL-12 and TNF-[alpha] production via adenosine A2a receptor-dependent and independent mechanisms." Faseb j 14(13): 2065-2074.
[5] Scott, G. S., et al. (2002). "Therapeutic intervention in experimental allergic encephalomyelitis by administration of uric acid precursors." Proc Natl Acad Sci U S A 99(25): 16303-16308.
[6] Kumar, V., & Sharma, A. (2009). "Adenosine: An endogenous modulator of innate immune system with therapeutic potential." European Journal of Pharmacology, 616(1–3), 7-15. doi: http://dx.doi.org/10.1016/j.ejphar.2009.05.005
[7] Wu, L.-F., et al. (2012). "Apoptosis induced by adenosine involves endoplasmic reticulum stress in EC109 cells." Int. J. Mol. Med. 30(4): 797-804.
[8] Cronstein, B. N., et al. (1985). "Adenosine; a physiologic modulator of superoxide anion generation by human neutrophils. Adenosine acts via an A2 receptor on human neutrophils." J Immunol 135(2): 1366-1371.
[9] Cronstein, B. N. (1994). "Adenosine, an endogenous anti-inflammatory agent." J Appl Physiol 76(1): 5-13.
[10] Flamand, N., et al. (2000). "Adenosine, a potent natural suppressor of arachidonic acid release and leukotriene biosynthesis in human neutrophils." Am J Respir Crit Care Med 161(2 Pt 2): S88-94.
[11] He, W., Mazumder, A., Wilder, T., & Cronstein, B. N. (2013). "Adenosine regulates bone metabolism via A1, A2A, and A2B receptors in bone marrow cells from normal humans and patients with multiple myeloma." FASEB J., 27(9), 3446-3454, 3410.1096/fj.3413-231233. doi: 10.1096/fj.13-231233
[12] Akula, K. K., Kaur, M., & Kulkarni, S. K. (2008). "Estimation of adenosine and its major metabolites in brain tissues of rats using high-performance thin-layer chromatography–densitometry." Journal of Chromatography A, 1209(1–2), 230-237. doi: http://dx.doi.org/10.1016/j.chroma.2008.08.086
[13] Kim, J., & Lee, J. (2011). "Observation and quantification of self-associated adenosine extracted from royal jelly products purchased in USA by HPLC." Food Chemistry, 126(1), 347-352. doi: http://dx.doi.org/10.1016/j.foodchem.2010.10.079
[14] Sottofattori, E., Anzaldi, M., & Ottonello, L. (2001). "HPLC determination of adenosine in human synovial fluid." Journal of Pharmaceutical and Biomedical Analysis, 24(5–6), 1143-1146. doi: http://dx.doi.org/10.1016/S0731-7085(00)00574-4
[15] Abdel-Hamid, M., Novotny, L., & Hamza, H. (2000). "Stability study of selected adenosine nucleosides using LC and LC/MS analyses." Journal of Pharmaceutical and Biomedical Analysis, 22(5), 745-755. doi: http://dx.doi.org/10.1016/S0731-7085(99)00276-9

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