化学名称 蝙蝠葛碱
外观 白色结晶粉末
化学物质登录号 524-17-4
分子式
分子量 624.76
植物来源
生物活性
鉴定 熔点 115°C
旋亮度 [α]17D-142°
UV
IR
ESI-MS
1HNMR
13CNMR
分析方法
色谱柱 Agilent TC-C18 色谱柱
流动相 A: 0.05% 乙腈, B: 三乙胺. 流率: 1.0 mL/min
检测器 UV λ284 nm
色谱柱 ZORBAX SB-C18 色谱柱 (4.6 mm × 250 mm, 5 μm)
流动相 A: 乙腈, B: H2O-甲酸 (100: 0.5)
检测器 UV λ268 nm
仪器 LC-MS 仪配备一个 G1311A 四元泵, G1379A 真空脱气机, G1313A 自动进样器, G1316A 柱加热器
色谱柱 Agilent TC-C18 色谱柱 (150 mm × 4.6 mm, 5 μm, Agilent, USA), 30°C
流动相 甲醇-水-冰醋酸 (60: 40: 0.8, v/v/v), 0.7 mL/min
检测器 Agilent API2000 三重四极杆质谱仪 (Agilent Corporation, MA, USA), ESI源, 正离子模式.离子喷雾: 5500 V, 加热气温: 450°C, 辅助器 (Gas 1): 60 psi, 加热气体 (Gas 2): 70 psi, 气帘: 10 psi, 碰撞气: 3 psi. 氮气.分布电压 (DP): 80 V. 多反映检测 (MRM): m/z 625.3-m/z 206.2
样品制备
方法一
TBE-300A HSCCC
石油醚-乙酸乙酯-乙醇-水 (1: 2: 1: 2, V /V /V /V), 固定相: 上有机相, 流动相: 下水相; 反相模型; 样品浓度: 20 mg/ml; 进样量: 20ml
2.0 mL/min, 850 rpm
HPLC, MS, NMR
参考文献
[1] Li, J. L., Dongfei. (2012). Determination of dauricine in Menispermi rhizoma by HPLC. Zhongguo Yaoshi, 26(10), 1122-1124.
[2] Wang, J., et al. (2012). "Dauricine can inhibit the activity of proliferation of urinary tract tumor cells." Asian Pac J Trop Med 5(12): 973-976.
[3] CHEN Wei, W. S.-x., HU Xiao-mao, XING Xiao-bo, CAI Ji-ye (2012). "Atomic Force Microscope-related Study on Dauricine Induced Apoptosis of NB4 Cell." 33(4): 421-428.
[4] Jin, H., et al. (2012). "CYP3A-mediated apoptosis of dauricine in cultured human bronchial epithelial cells and in lungs of CD-1 mice." Toxicol Appl Pharmacol 261(3): 248-254.
[5] Jin, H., et al. (2012). "CYP3A-mediated apoptosis of dauricine in cultured human bronchial epithelial cells and in lungs of CD-1 mice." Toxicology and Applied Pharmacology 261(3): 248-254.
[6] Zhao, J., et al. (2012). "Inhibitory effects of a bisbenzylisoquinline alkaloid dauricine on HERG potassium channels." Journal of Ethnopharmacology 141(2): 685-691.
[7] i, Y.-r. W., Ling-di; Zhang, Xiao-feng; Pan, Hai-feng. (2012). Research on HPLC fingerprint of Rhizoma Menispermi Zhongguo Shiyan Fangjixue Zazhi, 18(17), 127-130.
[8] Liu, X., et al. (2010). "Validated liquid chromatography-tandem mass spectrometry method for quantitative determination of dauricine in human plasma and its application to pharmacokinetic study." Journal of Chromatography B 878(15-16): 1199-1203.
[9] Luo, H. P., Aihua; Ye, Haoyu; Chen, Lijuan. (2009). Isolation and purification of alkaloid compounds from the roots of Menispermum dauricum using preparative high-speed counter-current chromatography. Sichuan Huagong, 34-37.
连结 中药材图像数据库 药用植物图像数据库 中药标本数据库

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