|
植物来源 |
|
|
生物活性 |
|
|
鉴定 |
熔点 |
328-332°C |
|
|
| 分析方法 |
|
| 仪器 |
硅胶板 60 F254 |
| 流动相 |
甲苯: 丙酮 = 2: 1 |
| 检测器 |
UV λ312 nm |
|
|
|
| 仪器 |
±30 kV 高压d.c. 电源提供毛细管电压, 毛细管进口为正电, 出口保持地面, 300μm碳盘工作电极口径三电极电化学池, 铂辅助电极, SCE参考电极 |
| 色谱柱 |
40 cm × 25 μm I.D. × 360 μm O.D. 硅胶熔融毛细管柱 |
| 流动相 |
50 mmol/l BB, pH 9.0 电泳缓冲液 |
| 检测器 |
BAS LC-4C 安培检测器. 过滤器0.1 Hz |
|
|
|
| 仪器 |
Waters Breeze 液相色谱仪配置 Waters 1525 二元高压泵和恒温柱 |
| 色谱柱 |
Agilent HC-C18 色谱柱 (250 mm × 4.6 mm i. d., 5.0 μm) 和保护柱 (4. 6 mm × 20 mm; packed with HC-C18 |
| 流动相 |
A: 乙腈, B: KH2PO4-三乙胺缓冲液 = pH 7.5, 0.01 M |
| 检测器 |
Waters 2996二极管数组检测器. Waters 2475 可变波长荧光检测器. |
|
|
|
| 仪器 |
CE-ED 仪及30 kV 高压电源 (Shanghai Institute of Nuclear Research, China), 口径 300 μm 碳盘工作电极 +900 mV |
| 色谱柱 |
长70 cm, 25 μm i.d., 360 μm o.d. 硅胶熔融毛细管柱 (Polymicro Technologies, Phoenix, AZ, USA). 18 kV 电动进样10 s. |
| 流动相 |
25 mmol/l H3BO3-Na2B4O7 缓冲液和 SDS, 0-25 mmol/l pH 7.6-8.7 |
| 检测器 |
BAS LC-4C 安培检测器 (Biochemical System, West Lafayette, IN, USA) |
|
| 样品制备 |
|
方法一 |
|
|
|
|
|
TBE-300A 仪 (Shenzhen Tauto Biotechnique Co. Ltd., Shenzhen, China) 三层盘绕线圈连接 (管径, 2.6 mm, 总体积 300 ml), 20 ml 手动进样回路. |
|
|
氯仿: 甲醇: 水 = 4: 3: 2 |
|
|
2 mL/min, 800 rpm |
|
|
UV λ254 nm |
|
|
| 参考文献 |
|
[1]
|
Jia, T.-L., et al. (2003). "Daidzein enhances osteoblast growth that may be mediated by increased bone morphogenetic protein (BMP) production." Biochemical Pharmacology 65(5): 709-715. |
|
[2]
|
Chen, G., et al. (2001). "Determination of puerarin, daidzein and rutin in Pueraria lobata (Wild.) Ohwi by capillary electrophoresis with electrochemical detection." Journal of Chromatography A 923(1–2): 255-262. |
|
[3]
|
Liu, Y.-K., et al. (2011). "Response surface optimization of EXTRACTION for HPLC-fluorescence determination of puerarin and daidzein in Radix Puerariae thomsonii." Journal of Pharmaceutical Analysis 1(1): 13-19. |
|
[4]
|
Gao, Y., et al. (2008). "The role of daidzein-loaded sterically stabilized solid lipid nanoparticles in therapy for cardio-cerebrovascular diseases." Biomaterials 29(30): 4129-4136. |
|
[5]
|
Choi, E. J. and G.-H. Kim (2008). "Daidzein causes cell cycle arrest at the G1 and G2/M phases in human breast cancer MCF-7 and MDA-MB-453 cells." Phytomedicine 15(9): 683-690. |
|
[6]
|
Zhang, H.-T., et al. (2010). "Daidzein relaxes rat cerebral basilar artery via activation of large-conductance Ca2+-activated K+ channels in vascular smooth muscle cells." European Journal of Pharmacology 630(1–3): 100-106. |
|
[7]
|
Siegelin, M. D., et al. (2009). "Daidzein overcomes TRAIL-resistance in malignant glioma cells by modulating the expression of the intrinsic apoptotic inhibitor, bcl-2." Neuroscience Letters 454(3): 223-228. |
|
[8]
|
Yokoyama, S. and T. Kuzuguchi (2007). "Rapid and convenient detection of urinary equol by thin-layer chromatography." J Nutr Sci Vitaminol (Tokyo) 53(1): 43-47. |
|
[9]
|
Cao, Y., et al. (2002). "Determination of puerarin and daidzein in Puerariae radix and its medicinal preparations by micellar electrokinetic capillary chromatography with electrochemical detection." Analytica Chimica Acta 452(1): 123-128. |
|
[10]
|
Qu, L., et al. (2006). "Extraction of daidzein and genistein from Semen Sojae Preparatum by high-speed counter-current chromatography." Zhongcaoyao 37(3): 375-377. |
|
| 连结 |
 中药材图像数据库
药用植物图像数据库
中药标本数据库
|
