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Natural Resources |
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Bioactivities |
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Identification |
Melting point |
132-135°C |
1HNMR
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| Analytical Method |
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| INSTRUMENT |
TLC plates (Silica gel coated) |
| MOBILE PHASE |
A: methanol, B: water (9: 1) |
| DETECTION |
Visualized under iodine vapours |
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| INSTRUMENT |
Hitachi L-7200 plus autosampler and a Hitachi sys-tem solvent module L-7100 pump, L-7455 diode array detector, AD-7000 Manager software |
| COLUMN |
Cosmosil 5 C18-AR-II column (250 × 4.6 mm; 5 μm) |
| MOBILE PHASE |
A: acetonitrile, B: 50 mM sodium phosphate buffer (pH 3.0) or water with different pHvalues that were adjusted with 0.1 N phosphoric acid., 1.0 mL/min |
| DETECTION |
UV λ270 nm |
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| INSTRUMENT |
Agilent (Palo Alto, CA, USA) 1100 LC system equipped with a G1311A binary pump, a G1322A vacuum degasser, a G1316A thermostatted column compartment, a G1314A variable wavelength UV-visible detector, and an HP 1100 series manual injector with 20μl fixed loop. |
| COLUMN |
Diamonsil C18 analytical column (200 mm × 4.6 mm i.d., 5μm particle size) |
| MOBILE PHASE |
A: methanol, B: 1% acetic acid aqueous solution (55: 45, V/V), 1 mL/min |
| DETECTION |
UV λ278 nm |
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| Sample Preparation |
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METHOD 1 |
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Samples were desiccated and ground into powder. 1.25 g of S. ningpoensis was put into 50 ml of distilled solvent spiked with 30 µg/ml of IS, then placed in the MO-2270M1 model microwave oven. Heated by the microwave at a power of 200-700 W for 1-6 min. A condenser with a continuous flow of cooling water was used to condense the solvent vapor in order to allow the solvent to repeatedly take part in the extraction process. The flask was cooled in the air. Subsequent to the cooling, the solvent was centrifuged at 1200 x g for 10 min and the clear supernatant solution (20 µl) was analyzed by HPLC. |
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METHOD 2 |
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HSCCC instrument (P.C. Inc., Potomac, MD 20854, USA), |
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Hexane: ethanol: acetonitrile: water = 10: 8: 1: 1, v/v |
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3.0 mL/min, 800 rpm |
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GC-MS analysis: Shimadzu GC-17A gas chromatograph fitted with a fused silica DB-5 (30 m × 0.32 mm I.D., 0.25 μm film thickness) capillary column with helium as the carrier gas at a flow rate of 1.0 mL/min, temperature, initial 100°C for 1 min, increased with a rate of 10°C/min to 250°C, maintained for 30 min. Shimadzu QP5000 mass-selective detector at 70 eV. |
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| Reference |
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[1]
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Patra, K., et al. (2012). "Amelioration of cyclophosphamide induced myelosuppression and oxidative stress by cinnamic acid." Chemico-Biological Interactions 195(3): 231-239. |
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[2]
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Huang, D.-W. and S.-C. Shen (2012). "Caffeic acid and cinnamic acid ameliorate glucose metabolism via modulating glycogenesis and gluconeogenesis in insulin-resistant mouse hepatocytes." Journal of Functional Foods 4(1): 358-366. |
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[3]
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Song, F., et al. "Protective effects of cinnamic acid and cinnamic aldehyde on isoproterenol-induced acute myocardial ischemia in rats." Journal of Ethnopharmacology (0). |
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[4]
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Kasetti, R. B., et al. (2012). "Cinnamic acid as one of the antidiabetic active principle(s) from the seeds of Syzygium alternifolium." Food and Chemical Toxicology 50(5): 1425-1431. |
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[5]
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Liu, J., et al. (2010). "Effects of Cinnamic Acid on Bacterial Community Diversity in Rhizosphere Soil of Cucumber Seedlings Under Salt Stress." Agricultural Sciences in China 9(2): 266-274. |
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[6]
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Lin, I., Jr., et al. (2010). "Simultaneous determination of hesperidin, ferulic acid, cinnamic acid and cinnamaldehyde in Chinese tonic wine by high performance liquid chromatography." J. Chin. Chem. Soc. (Taipei, Taiwan) 57(3A): 429-435. |
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[7]
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Huang, T., et al. (2011). "Rapid determination of cinnamic acid and harpagoside in a traditional Chinese medicine of Scrophularia ningpoensis by EXTRACTION followed by high performance liquid chromatography (HPLC)." J. Med. Plants Res. 5(8): 1313-1320. |
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[8]
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Severino, V. G. P., et al. (2009). "Isolation of secondary metabolites from Hortia oreadica (Rutaceae) leaves through high-speed counter-current chromatography." Journal of Chromatography A 1216(19): 4275-4281. |
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| Link to |
 Chinese Medicinal Material Images Database
Medicinal Plant Images Database
Chinese Medicine Specimen Database
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