化学名称 洛贝林
外观 白色至类白色结晶粉末
化学物质登录号 90-69-7
分子式
分子量 337.46
植物来源
生物活性
鉴定
1HNMR
13CNMR
分析方法
仪器 Agilent 1100 Series 系统 G1311A 四元泵及 G1313A 自动进样器
色谱柱 反相色谱柱 (Zorbax Extend-C18, 3.0 mm × 100 mm, 3.5 μm, Agilent, USA) 连接一个保护柱 (cartridge 2.1 mm × 12.5 mm, 5 μm, Agilent), 25°C
流动相 甲醇: 2.0 mmol/L 乙酸铵 = 70: 30 (v/v), 甲酸调节pH 3.5, 0.2 mL/min
检测器 LCQ 双重四极杆离子阱串联质谱 (Thermo-Finnigan, Corp., San Jose, USA), 正离子模式, 鞘气: 氮气 (80 arbitrary units), 源喷雾: −60 kV, 毛细管: −37 V, 加热毛细管: 250°C.
样品制备
方法一
水及乙酸提取两次, 3 小时. 过滤并用 10% 碳酸氢钠溶液碱化.
石油醚提取物以硫酸酸化. 10% 碳酸氢钠碱化. 干燥, 重溶解于水中, 稀盐酸稀释, 过滤, 氯仿震摇.
干燥的氯仿部以 60°C 水溶解, 真空干燥后留下水溶液部, 结晶得目标化合物. 重结晶纯化.
参考文献
[1] Tamboli, A. M., et al. (2012). "Antiepileptic activity of lobeline isolated from the leaf of Lobelia nicotianaefolia and its effect on brain GABA level in mice." Asian Pac. J. Trop. Biomed. 2(7): 537-542.
[2] Balvanyos, I., et al. (2004). "Analysis of polyacetylenes by HPLC in hairy root cultures of Lobelia inflata cultivated in bioreactor." Chromatographia 60(Suppl. 1): S235-S238.
[3] Kesting, J. R., et al. (2009). "Piperidine and tetrahydropyridine alkaloids from Lobelia siphilitica and Hippobroma longiflora." J Nat Prod 72(2): 312-315.
[4] Gu, M., et al. (2011). "Reversal effect of lobeline on multidrug-resistance of cancer cell." Xiandai Shengwuyixue Jinzhan 11(2): 378-380.
[5] Miller, D. K., et al. (2011). "Lobeline attenuates the locomotor-activating properties of repeated morphine treatment in rats." Trop. J. Pharm. Res. 10(4): 421-429.
[6] Smith, A. M., et al. (2012). "Lobeline attenuates neonatal ethanol-mediated changes in hyperactivity and dopamine transporter function in the prefrontal cortex in rats." Neuroscience (Amsterdam, Neth.) 206: 245-254.
[7] Tatsuta, T., et al. (2006). "Lobeline attenuates methamphetamine-induced stereotypy in adolescent mice." Neurochem Res 31(11): 1359-1369.
[8] Ruan, J., et al. (2011). "Clinical observation of lobeline for fentanyl intravenous anesthesia combined with propofol in painless gastroscopy." Shiyong Yixue Zazhi 27(10): 1852-1854.
[9] Colas, C., et al. (2012). "Lobeline docking on AChBP and nicotinic receptors: discriminating importance of the pocket geometry and of the ligand configuration." Lett. Drug Des. Discovery 9(1): 54-62.
[10] Jeong, I., et al. (2010). "Effects of lobeline, a nicotinic receptor ligand, on the cloned Kv1.5." Pfluegers Arch. 460(5): 851-862.
[11] Dimatelis, J. J., et al. (2012). "The effects of lobeline and naltrexone on methamphetamine-induced place preference and striatal dopamine and serotonin levels in adolescent rats with a history of maternal separation." Metab. Brain Dis. 27(3): 351-361.
[12] Song, W., et al. (2008). "Identification of metabolites of lobeline in the rat urine by liquid chromatography-tandem mass spectrometry." International Journal of Mass Spectrometry 269(1-2): 131-137.

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