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植物来源 |
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|
生物活性 |
|
|
鉴定 |
熔点 |
166°C |
| 旋亮度 |
[α]25D-0.21° (c, 17.6 水), [α]25D+28.61° (c, 10 in 12.8% 硼水) |
1HNMR
|
| 分析方法 |
|
| 仪器 |
Hewlett-Packard 毛细管GLC仪 (model5880A) |
| 色谱柱 |
GB-IWCOT 毛细管柱 (25m × 25 mm i.d., The Foxboro Co., Foxboro, MA) |
| 流动相 |
温度程序: 3 min 150°C, 5°C min-1 至 280°C, 280°C 3min. |
| 检测器 |
电子碰撞模式: 70-900 amu/2s. DB-5 硅胶熔融毛细管柱 (15m x 0.32mm I.D., 膜厚度0.25Ixm, J &W Scientific, Folsom, CA) 分流进样 (10: 1), 载气: 氦气 (30 cm s-1线速度). |
|
|
|
| 仪器 |
PerkinElmer Spectrum™ 100 FTIR 波普仪 |
| 检测器 |
频率范围: 4000-650 cm-1, 4 cm-1 收集 Strong Beer-Norton 切趾法分辨率 |
|
|
|
| 仪器 |
Hewlett Packard HP1090 仪配备HP ChemStation 数据处理软件 (Rev. A.03.03), HP Vectra 4DX66 PC |
| 色谱柱 |
Dionex Carbopac PA-100/ PA-10 分析色谱柱 (250 × 4 mm), Dionex Carbopac PA-100 或氨基阱保护柱 |
| 流动相 |
0.1 M NaOH, 1.0 mL/min |
|
|
|
| 色谱柱 |
CarboPac PA1 保护柱 (Dionex P/N 43096; 4 × 50 mm), CarboPac PA1 阴离子交换分析色谱柱 (Dionex P/N 35391; 4 × 250 mm) |
| 流动相 |
A: 100 mM NaOH, B: milliQ 水, C : 100mM NaOH +1 M 乙酸钠: 0-7 min 10% A 90% B, 7-20 mins 50% A 50% B, 20-45 min 10% A 50% B 40% C, 45-45.1 min 50% A 50% B, 45.1-60 min 50% A 50% B, 60-60.1 min 10% A 90% B, 60.1-70 min 10% A 90% B: 流率: 1 mL/min |
| 检测器 |
UV λ215 nm |
|
| 参考文献 |
|
[1]
|
Ko, R. K., et al. (2013). "Anti-melanogenesis constituents from the seaweed Dictyota coriacea." Nat Prod Commun 8(4): 427-428. |
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[2]
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Schmatz, D. M., et al. (1988). "Identification and possible role of d-mannitol and 2-O-methyl-chiroinositol (quebrachitol) in Eimeria tenella." Molecular and Biochemical Parasitology 29(1): 29-36. |
|
[3]
|
Ceccaroli, P., et al. (2007). "Identification and characterization of the Tuber borchii d-mannitol dehydrogenase which defines a new subfamily within the polyol-specific medium chain dehydrogenases." Fungal Genetics and Biology 44(10): 965-978. |
|
[4]
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Min, X., et al. (2013). "Effect of D-mannitol on nitrogen retention, fiber digestibility and digesta transit time in adult rabbits." Anim. Sci. J. (Richmond, Aust.) 84(7): 551-555. |
|
[5]
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Xiao, J., et al. (2013). "Mannitol improves absorption and retention of calcium and magnesium in growing rats." Nutrition 29(1): 325-331. |
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[6]
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Tang, S.-C., et al. "Effect of mannitol on cerebrovascular pressure reactivity in patients with intracranial hypertension." Journal of the Formosan Medical Association (0). |
|
[7]
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Ye, P. and T. Byron (2008). "Characterization of D-mannitol by thermal analysis, FTIR, and Raman spectroscopy." Am. Lab. (Shelton, CT, U. S.) 40(14): 24-27. |
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[8]
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Wring, S. A., et al. (1998). "The electroanalysis of mannitol, xylose and lactulose at copper electrodes: voltammetric studies and bioanalysis in human urine by means of HPLC with electrochemical detection." Journal of Pharmaceutical and Biomedical Analysis 16(7): 1213-1224. |
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[9]
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Wannet, W. J. B., et al. (2000). "HPLC Detection of Soluble Carbohydrates Involved in Mannitol and Trehalose Metabolism in the Edible Mushroom Agaricus bisporus." J. Agric. Food Chem. 48(2): 287-291. |
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