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植物來源 |
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生物活性 |
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鑑定 |
熔點 |
171-175°C |
| 旋光度 |
[α]20D+84.8° (C=0.006g/100ml, 甲醇) |
1HNMR
|
| 分析方法 |
|
| 儀器 |
矽膠60 F254 TLC 板 (Merck, Germany) |
| 流動相 |
正丁醇: 甲苯: 乙酸乙酯: 甲酸 = 2: 5: 2.5: 0.5 |
| 檢測器 |
UV λ254 nm, UV λ366 nm |
|
|
|
| 儀器 |
Agilent 1100 HPLC 儀, 包括四元梯度泵, DAD檢測器和自動進樣器 (0.1-100 μL) |
| 色譜柱 |
Grace Alltima-C18 色譜柱 (250 mm × 4.6 mm, 5 μm, Phenomenex, USA). |
| 流動相 |
A: 0.1% (v/v)甲酸-水, B: 乙腈, 0-30 min 88% A, 30-45min 80% A, 45-60min 70% A, 1.0 mL/min |
| 檢測器 |
UV λ330 nm |
|
|
|
| 儀器 |
HP 1100 系列 (Agilent Technologies, Waldbronn, Germany), 包括四元梯度泵, 線上脫氣機, 自動進樣器, 柱溫箱和 DAD檢測器. |
| 色譜柱 |
Tigerkin C18 色譜柱 (200 mm × 4.6 mm i.d., 5.0 µm) 購自大連思譜精工有限公司 (大連, 中國) |
| 流動相 |
A: 0.5% (v/v) 甲酸-水, B: 0.5% (v/v) 乙腈-甲酸, 0-10 min: 5-20% B, 10-17 min: 20-25% B, 17-35 min: 25-55% B, 1.0 mL/min |
| 檢測器 |
UV λ326 nm |
|
| 樣品製備 |
|
方法一 |
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|
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TBE 300A HSCCC |
|
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石油醚-乙酸乙酯-甲醇-0.5% 甲酸水 (2: 5: 2: 5) |
|
|
2.0 mL/min, 800 r/min |
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TLC 檢測, 展開劑為石油醚: 乙酸乙酯 = 1: 1, 氯仿: 甲醇: 水 = 65: 35: 10 (下層), 正丁醇: 冰醋酸: 水 = 4: 1: 5 (上層) 顯色劑: 10% 硫酸乙醇溶液, ESI-MS 檢測, [M+Na]+m/z383 |
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方法二 |
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0.25 g 冷乾粉溶于水 (20 ml, pH 3), 倒於 Amberlite XAD-7柱 (直徑 12 mm, 高 9-10 mm, 樹脂重 10 g), 用水 (15 ml, pH 3) 80% 的乙醇水溶液洗脫 |
|
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0.5 g RA 粗提物冷乾粉溶于10 ml 水 (pH 3) 和 0.5 g NaCl, 用乙酸乙酯提取 3 次, 每次 20 ml, 合併乙酸乙酯提取液 (經無水硫酸鈉脫水後), 濃縮至幹, 殘留物溶於乙醇 |
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0.5 g RA 粗提物冷乾粉溶於 10 ml 水, 用飽和的氫氧化鈣溶液調至 pH 10, 將迷迭香酸鈣鹽沉澱物 5000 g 離心 20 分鐘, 測定上清液中殘留的迷迭香酸, 用適量氫氧化鈣重複上述實驗對上清液進行第二次沉澱 |
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| 參考文獻 |
|
[1]
|
Liu, J., et al. (2013). "Determination of the content of rosmarinic acid by HPLC and analytical comparison of volatile constituents by GC-MS in different parts of Perilla frutescens (L.) Britt." Chemistry Central Journal 7(1): 61. |
|
[2]
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Oliveira, K. B., et al. (2013). "Influence of rosmarinic acid and Salvia officinalis extracts on melanogenesis of B16F10 cells." Revista Brasileira de Farmacognosia 23: 249-258. |
|
[3]
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Li, Y., et al. (2012). "On-line antioxidant activity determination of main ingredients in Guan-Xin-Ning injection by HPLC-DAD-CL." Chinese Journal of Natural Medicines 10(6): 448-455. |
|
[4]
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Baliga, M. S., et al. (2013). "Ocimum sanctum L (Holy Basil or Tulsi) and its phytochemicals in the prevention and treatment of cancer." Nutr Cancer 65 Suppl 1: 26-35. |
|
[5]
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Xu, J. Z., et al. (2008). "Simultaneous detection of seven phenolic acids in Danshen injection using HPLC with ultraviolet detector." J Zhejiang Univ Sci B 9(9): 728-733. |
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[6]
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Huang, L., et al. (2011). "High-speed counter-current chromatography separation and purification of rosmarinic acid from Perilla frutescens (L.) Britt. var. acuta (Thunb.) Kudo form. discolor (Makino) Kudo." Yaowu Fenxi Zazhi 31(11): 2087-2090. |
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[7]
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Georgiev, M., et al. (2006). "Purification of rosmarinic acid extracts from Lavandula vera MM cell biomass." Food Chemistry 94(1): 111-114. |
|
[8]
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Gu, L., et al. (2009). "TLC bioautography-guided isolation of antioxidants from fruit of Perilla frutescens var. acuta." LWT - Food Science and Technology 42(1): 131-136. |
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| 連結 |
 中藥材圖像數據庫
藥用植物圖像數據庫
中藥標本數據庫
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