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植物来源 |
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生物活性 |
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鉴定 |
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| 分析方法 |
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| 仪器 |
硅胶60 F254, 0.5 mm, 20 × 20 cm |
| 流动相 |
己烷: 丙酮 = 7: 3, v/v |
| 检测器 |
254 nm |
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| 仪器 |
Agilent Series 1100 液相色谱 |
| 色谱柱 |
A ZORBAX ODS C18 色谱柱 (4.6 mm × 250 mm i.d., 5 µm) 和 ZORBAX ODS C18 保护柱 (4.6 mm × 12.5 mm i.d., 5 µm) |
| 流动相 |
A: 1% 醋酸水溶液, B: 乙腈: 0-10 min 5-35% B, 10-30 min 35-50% B, 30-35 min 50-70% B, 35-50min 5% B 等度, 1.0 mL/min |
| 检测器 |
UV λ284 nm |
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| 仪器 |
HP 1090 Series II LC 系统 (Hewlett-Packard, Palo Alto, CA, USA) |
| 色谱柱 |
Waters Symmetry C18 色谱柱 (5 µm, 150 × 2.1 mm) 和保护柱 (Symmetry C18, 5 µm, 20 × 3.9 mm) |
| 流动相 |
A: 水 (0.25% HAc), B: 甲醇: 0-40 min 35-100% B, 40-42 min 100-35% B ; 流速: 0.2 mL/min |
| 检测器 |
正离子模式, 干燥气 (氮气): 350°C, 流速 40 mL/min, 雾化气压 (N2) : 5.5-105 Pa (80 p.s.i.) |
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| 仪器 |
Waters Temperature Control Module II, 717 plus 自动进样器, 2487 Dual λ 吸收光检测器, 515 HPLC 泵 |
| 色谱柱 |
Hedera ODS-2 色谱柱 (250 mm × 4.6 mm i.d., particle size 5 µm, Jiangsu Hanbang, China) |
| 流动相 |
甲醇: 水: 乙酸 = 68: 31.7: 0.3, v/v/v; 流速: 1.0 mL/min |
| 检测器 |
UV λ322 nm |
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| 仪器 |
ACQUITY UPLC 系统 (Waters Corp., Milford, MA, USA) 自动进样器 4°C |
| 色谱柱 |
ACQUITY UPLC HSS T3 色谱柱 (100 mm × 2.1 mm i.d., 1.8 µm; Waters Corp., Milford, MA, USA) |
| 流动相 |
A: 乙腈, B : 水 (含0.1% 甲酸): 0-1 min 2% A, 1-2 min 2-70% A, 2-4 min 70% A, 4-4.5min 70-2% A, 4.5-5.5 min 2% A; 流速: 2.0 µL/min |
| 检测器 |
正离子模式, 碰撞电压: 3.0 kV. 离子源和去溶剂温度: 100°C 和 300°C, 锥气和去溶剂 : 50 L h− 1 和600 L h− 1, SIM 模式, m/z: 191.107. |
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| 仪器 |
Shimadzu LC-20AVP 系统, 两个 LC-20AT 溶剂单元, SPD-M20AVP UV-visPAD 检测器 |
| 色谱柱 |
Apollo C18 色谱柱 (150 × 4.6 mm I.D., 5 µm) |
| 流动相 |
A: 甲醇, B: 0.05% H3PO4: 0-3 min 45% B, 3-17 min 40% B, 17-43 min 33% B; 流速: 1 mL/min |
| 检测器 |
UV λ254 nm |
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| 样品制备 |
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方法一 |
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Model GS-20 A 分析多层螺旋管行星式离心机包括 PTFE 多层线圈 70 m × 0.85 mm I.D. 总容量 40 m |
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正己烷: 乙酸乙酯: 甲醇: 水: 乙腈 = 8: 2: 5: 5: 5, v/v |
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1.0 mL/min, 1600 rpm |
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UV λ254 nm |
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| 参考文献 |
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[1]
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Li, P., et al. (2006). "Optimization of pressurized liquid extraction for Z-ligustilide, Z-butylidenephthalide and ferulic acid in Angelica sinensis." Journal of Pharmaceutical and Biomedical Analysis 40(5): 1073-1079. |
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[2]
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Cheng, L.-L., et al. (2011). "Z-ligustilide isolated from Radix Angelicae sinensis ameliorates the memory impairment induced by scopolamine in mice." Fitoterapia 82(7): 1128-1132. |
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[3]
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Lager, E., et al. (2007). "Diels-Alder adducts derived from the natural phthalide Z-ligustilide." Tetrahedron Letters 48(24): 4215-4218. |
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[4]
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Li, P., et al. (2006). "Optimization of pressurized liquid extraction for Z-ligustilide, Z-butylidenephthalide and ferulic acid in Angelica sinensis." Journal of Pharmaceutical and Biomedical Analysis 40(5): 1073-1079. |
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[5]
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Peng, B., et al. (2013). "Z-ligustilide activates the Nrf2/HO-1 pathway and protects against cerebral ischemia-reperfusion injury in vivo and in vitro." Brain Research 1520(0): 168-177. |
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[6]
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Cao, Y.-X., et al. (2006). "Ligustilide induces vasodilatation via inhibiting voltage dependent calcium channel and receptor-mediated Ca2+ influx and release." Vascular Pharmacology 45(3): 171-176. |
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[7]
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Chan, S. S.-K., et al. (2007). "Relaxation effects of ligustilide and senkyunolide A, two main constituents of Ligusticum chuanxiong, in rat isolated aorta." Journal of Ethnopharmacology 111(3): 677-680. |
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[8]
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Su, Y.-W., et al. (2011). "Ligustilide prevents LPS-induced iNOS expression in RAW 264.7 macrophages by preventing ROS production and down-regulating the MAPK, NF-κB and AP-1 signaling pathways." International Immunopharmacology 11(9): 1166-1172. |
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[9]
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Lin, L.-Z., et al. (1998). "Liquid chromatographic-electrospray mass spectrometric study of the phthalides of Angelica sinensis and chemical changes of Z-ligustilide." Journal of Chromatography A 810(1-2): 71-79. |
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[10]
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Guo, J., et al. (2009). "Determination of ligustilide in rat brain after nasal administration of essential oil from Rhizoma Chuanxiong." Fitoterapia 80(3): 168-172. |
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[11]
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Guo, J., et al. (2011). "Determination of ligustilide in the brains of freely moving rats using microdialysis coupled with ultra performance liquid chromatography/mass spectrometry." Fitoterapia 82(3): 441-445. |
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[12]
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Wei, Y., et al. (2013). "Online isolation and purification of four phthalide compounds from Chuanxiong rhizoma using high-speed counter-current chromatography coupled with semi-preparative liquid chromatography." Journal of Chromatography A 1284(0): 53-58. |
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| 连结 |
 中药材图像数据库
药用植物图像数据库
中药标本数据库
|
