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| 仪器 |
Waters Acquity™ UPLC 仪 (Waters, Milford, USA) 配置一个四极泵, 真空脱气机, 自动进样器, 二极管数组检测器 |
| 色谱柱 |
ACQUITY UPLC BEH C18 色谱柱 (2.1 mm x 50 mm, 1.7 mm), 45°C |
| 流动相 |
A: 水, B: 甲醇, 0-1.0 min 5-40% B, 1.0-3.5 min 40-65% B, 3.5-4.0 min 65-70% B, 4.0-4.2 min 70-90% B, 4.2-5.0 min 90% B. |
| 检测器 |
Waters Acquity™ UPLC 仪配置一个ESI离子源, 正和负离子模式操作. 毛细管电压: 正离子模式3200 V, 负离子模式2500 V, 去溶剂化: 300°C, 样品锥: 30 V, 提取锥: 4 V, 源: 110°C, 锥气: 50 L/h, 去溶剂气: 600 L/h, 碰撞能: 正离子模式 3 eV, 负离子模式 2.5 eV, 碰撞气: 2.8 x 10-3mbar argon. |
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| 参考文献 |
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[1]
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Son, K. H., et al. (1999). "Isolation and quantitative determination of anemarsaponin B from the rhizomes of Anemarrhena asphodeloides Bunge." Saengyak Hakhoechi 30(2): 163-167. |
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[2]
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Ma, B., et al. (1996). "Studies on the furostanol saponins from Anemarrhena asphodeloides Bunge." Yaoxue Xuebao 31(4): 271-277. |
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[3]
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Kim, J.-Y., et al. (2009). "Anti-inflammatory effect of anemarsaponin B isolated from the rhizomes of Anemarrhena asphodeloides in LPS-induced RAW 264.7 macrophages is mediated by negative regulation of the nuclear factor-κB and p38 pathways." Food and Chemical Toxicology 47(7): 1610-1617. |
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[4]
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Nakashima, N., et al. (1993). "Isolation of pseudoprototimosaponin AIII from rhizomes of Anemarrhena asphodeloides and its hypoglycemic activity in streptozotocin-induced diabetic mice." J. Nat. Prod. 56(3): 345-350. |
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[5]
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Effects of Anemarrhena asphodeloides on focal ischemic brain injury induced by middle cerebral artery occlusion in rats. |
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[6]
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Antiosteoporotic chemical constituents from Er-Xian Decoction, a traditional Chinese herbal formula |
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[7]
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Dong, J. X. and G. Y. Han (1992). "Active constituentes of Anemarrhena asphodeloides Bunge." Yaoxue Xuebao 27(1): 26-32. |
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[8]
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A new active steroidal saponin from Anemarrhena asphodeloides |
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[9]
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Son, K. H., et al. (1999). "Isolation and quantitative determination of anemarsaponin B from the rhizomes of Anemarrhena asphodeloides Bunge." Saengyak Hakhoechi 30(2): 163-167. |
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[10]
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Yang, B., et al. (2011). "Ultra-performance liquid chromatography coupled with electrospray ionization/quadrupole-time-of-flight mass spectrometry for rapid analysis of constituents of Suanzaoren decoction." J Sep Sci 34(22): 3208-3215. |
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中药材图像数据库
