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植物來源 |
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生物活性 |
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鑑定 |
熔點 |
173.5-175.5°C |
| 旋光度 |
[α]D -78.8 (c, 1 氯仿) |
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| 分析方法 |
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| 儀器 |
Agilent 1200 HPLC 儀 (Agilent Technologies, Waldbronn, Germany) |
| 色譜柱 |
Zorbax Eclipse Plus C18 色譜柱 (1.8 μm, 4.6 × 150 mm, Agilent) |
| 流動相 |
A: 0.2% 甲酸, B: 乙腈, 0–1.5 min 5% B, 1.5–26 min 5- 95% B, 26–35 min 95% B, 1.4 mL/min |
| 檢測器 |
UV λ240 nm, 負離子模式MS, 毛細管電壓4000 V, 乾燥氣溫度350°C, 乾燥氣12 l/min, 噴霧器壓力45 psi, 碰撞誘導解離電壓140 V, 質量範圍100–2000 m/z. |
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| 儀器 |
Waters Acquity UPLC 儀 (Waters, Milford, MA, USA), 裝備一個二元溶劑輸送系統, 一個自動進樣器, 一個二極管數組檢測器 |
| 色譜柱 |
Waters Acquity BEH C8 色譜柱 (2.1 × 100 mm I.D., 1.7 μm, Waters, Milford, MA, USA) |
| 流動相 |
A: 0.1% 甲酸水: 甲醇 = 80: 20, B: 含 0.1% 甲酸乙腈 ACN, 0-8 min 30-45% 8-12 min B, 45-90% B, 12-13 min 90% B, 13-14 min 90-30% B, 0.5 mL/min |
| 檢測器 |
UV λ280 nm, 正離子模式, 霧化氣 600 L/h 霧化氣溫度 300°C, 錐氣 50 L/h, 離子源溫度 100°C. 毛細管電壓及錐電壓為 2700 V 和 35 V |
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| 樣品製備 |
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方法一 |
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乾燥粉末用 70% 乙醇室溫下提取。過濾, 濃縮, 並利用 30% 乙醇溶解, 並用正己烷、氯仿、正丁醇分段提取。 |
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正己烷提取物進矽膠色譜柱按己烷: 丙酮 = 10: 1 至純丙酮梯度分離15個餾分。 |
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餾分15 進矽膠色譜柱按丙酮: 己烷 = 1: 20 的比例得到目標化合物。 |
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| 參考文獻 |
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[1]
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Babovic, N., et al. (2010). "Supercritical carbon dioxide extraction of antioxidant fractions from selected Lamiaceae herbs and their antioxidant capacity." Innovative Food Science & Emerging Technologies 11(1): 98-107. |
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[2]
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Ivanovic, J., et al. (2009). "Supercritical carbon dioxide extraction of antioxidants from rosemary (Rosmarinus officinalis L.) and sage (Salvia officinalis L.)." J. Serb. Chem. Soc. 74(7): 717-732. |
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[3]
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Guerrero, I. C., et al. (2006). "Abietane Diterpenoids from Salvia pachyphylla and S. clevelandii with Cytotoxic Activity against Human Cancer Cell Lines." J. Nat. Prod. 69(12): 1803-1805. |
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[4]
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Escuder, B., et al. (2002). "Antioxidant capacity of abietanes from Sphacele salviae." Nat. Prod. Lett. 16(4): 277-281. |
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[5]
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Ahmed, A. A., et al. (1995). "Abietane diterpenes from Lepechinia urbaniana." Pharmazie 50(4): 279-280. |
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[6]
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Luis, J. G., et al. (1994). "Columbaridione, a diterpene quinone from Salvia columbariae." Phytochemistry 36(1): 251-252. |
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[7]
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Ulubelen, A., et al. (1992). "Diterpenoids from Salvia candidissima." Tetrahedron Lett. 33(47): 7241-7244. |
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[8]
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Kelecom, A. (1984). "An abietane diterpene from the labiate Coleus barbatus." Phytochemistry 23(8): 1677-1679. |
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[9]
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Mothana, R. A., et al. (2014). "In vitro antiprotozoal activity of abietane diterpenoids isolated from Plectranthus barbatus Andr." Int J Mol Sci 15(5): 8360-8371. |
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[10]
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Zhou, Y., et al. (2009). "Qualitative and quantitative analysis of diterpenoids in Salvia species by liquid chromatography coupled with electrospray ionization quadrupole time-of-flight tandem mass spectrometry." Journal of Chromatography A 1216(24): 4847-4858. |
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