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植物來源 |
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|
生物活性 |
|
|
鑑定 |
熔點 |
87.5°C |
1HNMR
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| 分析方法 |
|
| 儀器 |
矽膠G板 |
| 流動相 |
石油醚60-90°C |
| 檢測器 |
噴淋5% 香草醛硫酸溶液顯色 |
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|
|
| 儀器 |
LC-10Avp 泵, SPD-10Avp UV-VIS檢測器, SCL-10Avp 系統控制器 (Shimadzu, Kyoto, Japan) 和整合器 (SSC-982, Shanghai, China) |
| 色譜柱 |
Cosmosil 5 C18色譜柱, 4.6 × 150 mm, 5 μm (Nacalai Tesque, Inc, Kyoto, Japan) |
| 流動相 |
甲醇: 二氯甲烷: 水: 磷酸 = 81: 3: 16: 0.12, v/v/v/v, 0.5 mL/min |
| 檢測器 |
UV λ290 nm |
|
|
|
| 儀器 |
Waters Acquity UPLC 系統 (Waters Co., Milford, USA); Waters Q-ToF Premier 檢測器 (Micromass MS Technologies, Manchester, UK) |
| 色譜柱 |
Waters Acquity HSS T3 色譜柱 (2.1 mm × 100 mm, 1.8 μm, Wexford, Ireland, 10°C) |
| 流動相 |
A: 0.1% 甲酸水, B: 乙腈0.1% 甲酸, 0-6 min 5-15.9% B, 6-10 min 15.9-40% B, 10-15 min 40-60.5% B, 15-20 min 60-100% B |
|
| 樣品製備 |
|
方法一 |
|
|
厚朴樣品 (Magnolia officinalis Rehd. et Wils.) 150 mg, 兩相溶劑系統分離正己烷-乙酸乙酯-甲醇-水 (1: 0.4: 1: 0.4, v/v) |
|
|
利用高效液相色譜分離出 80 和 45 mg 和厚朴酚純度 99.2 |
|
|
| 參考文獻 |
|
[1]
|
Yu-Chiang, L., et al. (1994). "Magnolol and honokiol isolated from magnolia officinalis protect rat heart mitochondria against lipid peroxidation." Biochemical Pharmacology 47(3): 549-553. |
|
[2]
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Alonso-Castro, A. J., et al. (2011). "Magnolia dealbata Zucc and its active principles honokiol and magnolol stimulate glucose uptake in murine and human adipocytes using the insulin-signaling pathway." Phytomedicine 18(11): 926-933. |
|
[3]
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Park, E.-J., et al. (2009). "Down-regulation of c-Src/EGFR-mediated signaling activation is involved in the honokiol-induced cell cycle arrest and apoptosis in MDA-MB-231 human breast cancer cells." Cancer Letters 277(2): 133-140. |
|
[4]
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Tang, X., et al. (2011). "Honokiol inhibits H2O2-induced apoptosis in human lens epithelial cells via inhibition of the mitogen-activated protein kinase and Akt pathways." European Journal of Pharmacology 650(1): 72-78. |
|
[5]
|
Yin, H.-Q., et al. (2009). "Honokiol reverses alcoholic fatty liver by inhibiting the maturation of sterol regulatory element binding protein-1c and the expression of its downstream lipogenesis genes." Toxicology and Applied Pharmacology 236(1): 124-130. |
|
[6]
|
Liu, H., et al. (2008). "Anti-tumor effect of honokiol alone and in combination with other anti-cancer agents in breast cancer." European Journal of Pharmacology 591(1–3): 43-51. |
|
[7]
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Han, G.-R., et al. (2005). "Studies of TLC and quantitative determination of Kai Wei Wan." Jiefangjun Yaoxue Xuebao 21(6): 453-455. |
|
[8]
|
Wu, X., et al. (2003). "High-performance liquid chromatographic method for simultaneous determination of honokiol and magnolol in rat plasma." Talanta 59(1): 115-121. |
|
[9]
|
Zhou, W.-J., et al. (2013). "Chemical comparison of two dosage forms of Hemp Seed Pills by UPLC-Q-ToF–MS/MS and multivariate statistical techniques." Journal of Pharmaceutical and Biomedical Analysis 84(0): 59-68. |
|
[10]
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Wang, X., et al. (2004). "Isolation and purification of honokiol and magnolol from cortex Magnoliae officinalis by high-speed counter-current chromatography." Journal of Chromatography A 1036(2): 171-175. |
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| 連結 |
 中藥材圖像數據庫
藥用植物圖像數據庫
中藥標本數據庫
|
