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植物来源 |
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生物活性 |
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鉴定 |
熔点 |
87.5°C |
1HNMR
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| 分析方法 |
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| 仪器 |
硅胶G板 |
| 流动相 |
石油醚60-90°C |
| 检测器 |
喷淋5% 香草醛硫酸溶液显色 |
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| 仪器 |
LC-10Avp 泵, SPD-10Avp UV-VIS检测器, SCL-10Avp 系统控制器 (Shimadzu, Kyoto, Japan) 和整合器 (SSC-982, Shanghai, China) |
| 色谱柱 |
Cosmosil 5 C18色谱柱, 4.6 × 150 mm, 5 μm (Nacalai Tesque, Inc, Kyoto, Japan) |
| 流动相 |
甲醇: 二氯甲烷: 水: 磷酸 = 81: 3: 16: 0.12, v/v/v/v, 0.5 mL/min |
| 检测器 |
UV λ290 nm |
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| 仪器 |
Waters Acquity UPLC 系统 (Waters Co., Milford, USA); Waters Q-ToF Premier 检测器 (Micromass MS Technologies, Manchester, UK) |
| 色谱柱 |
Waters Acquity HSS T3 色谱柱 (2.1 mm × 100 mm, 1.8 μm, Wexford, Ireland, 10°C) |
| 流动相 |
A: 0.1% 甲酸水, B: 乙腈0.1% 甲酸, 0-6 min 5-15.9% B, 6-10 min 15.9-40% B, 10-15 min 40-60.5% B, 15-20 min 60-100% B |
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| 样品制备 |
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方法一 |
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厚朴样品 (Magnolia officinalis Rehd. et Wils.) 150 mg, 两相溶剂系统分离正己烷-乙酸乙酯-甲醇-水 (1: 0.4: 1: 0.4, v/v) |
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|
利用高效液相色谱分离出 80 和 45 mg 和厚朴酚纯度 99.2 |
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| 参考文献 |
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[1]
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Yu-Chiang, L., et al. (1994). "Magnolol and honokiol isolated from magnolia officinalis protect rat heart mitochondria against lipid peroxidation." Biochemical Pharmacology 47(3): 549-553. |
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[2]
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Alonso-Castro, A. J., et al. (2011). "Magnolia dealbata Zucc and its active principles honokiol and magnolol stimulate glucose uptake in murine and human adipocytes using the insulin-signaling pathway." Phytomedicine 18(11): 926-933. |
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[3]
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Park, E.-J., et al. (2009). "Down-regulation of c-Src/EGFR-mediated signaling activation is involved in the honokiol-induced cell cycle arrest and apoptosis in MDA-MB-231 human breast cancer cells." Cancer Letters 277(2): 133-140. |
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[4]
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Tang, X., et al. (2011). "Honokiol inhibits H2O2-induced apoptosis in human lens epithelial cells via inhibition of the mitogen-activated protein kinase and Akt pathways." European Journal of Pharmacology 650(1): 72-78. |
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[5]
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Yin, H.-Q., et al. (2009). "Honokiol reverses alcoholic fatty liver by inhibiting the maturation of sterol regulatory element binding protein-1c and the expression of its downstream lipogenesis genes." Toxicology and Applied Pharmacology 236(1): 124-130. |
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[6]
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Liu, H., et al. (2008). "Anti-tumor effect of honokiol alone and in combination with other anti-cancer agents in breast cancer." European Journal of Pharmacology 591(1–3): 43-51. |
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[7]
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Han, G.-R., et al. (2005). "Studies of TLC and quantitative determination of Kai Wei Wan." Jiefangjun Yaoxue Xuebao 21(6): 453-455. |
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[8]
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Wu, X., et al. (2003). "High-performance liquid chromatographic method for simultaneous determination of honokiol and magnolol in rat plasma." Talanta 59(1): 115-121. |
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[9]
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Zhou, W.-J., et al. (2013). "Chemical comparison of two dosage forms of Hemp Seed Pills by UPLC-Q-ToF–MS/MS and multivariate statistical techniques." Journal of Pharmaceutical and Biomedical Analysis 84(0): 59-68. |
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[10]
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Wang, X., et al. (2004). "Isolation and purification of honokiol and magnolol from cortex Magnoliae officinalis by high-speed counter-current chromatography." Journal of Chromatography A 1036(2): 171-175. |
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| 连结 |
 中药材图像数据库
药用植物图像数据库
中药标本数据库
|
