化學名稱 白藜蘆醇
外觀 微黃的白色粉末
化學物質登錄號 501-36-0
分子式
分子量 228.24
植物來源
生物活性
鑑定 熔點 253-255°C
旋光度 [α]20D-18°至-22°
1HNMR
分析方法
儀器 矽膠60 板
流動相 正己烷-乙酸乙酯-甲酸 =20: 19: 1 (v/v)
檢測器 UV λ303 nm
儀器 Agilent 1200 液相色譜系統 (Agilent Technologies, Germany) 配備二極管數組檢測器 (DAD G1315D), model G1322A 脫氣機, G1311A 四元泵及 G1316A 自動進樣器.
色譜柱 C18 色譜柱 (AichromBond 5 μm, 250 × 4.60 mm)
流動相 A: 乙腈, B: 0.2% 甲酸水溶液, 25: 75, 0.8 mL/min
檢測器 UV λ306 nm
儀器 Merck-Hitachi L-6200 泵 (Merck-Hitachi, Darmstadt, Germany)及二極體陣列檢測器Shimadzu SPD-M6A UV (Shimadzu, Kyoto, Japan).
色譜柱 Agilent Zorbax Bioseries GF-450 色譜柱 (Santa Clara, USA) (250 × 9.4 mm I.D. 6 μm)
流動相 流動相 (0.2 M 醋酸鈉 pH 4.0, 0.2 M 磷酸鈉 pH 7.0-8.5 和 0.2 M 硼酸鈉 pH 9.0-10.0), α-, β- 或 γ-CD 計入 250 mL 溶劑. 達到環境溫度, 計入剩餘溶劑. (Chatjigakis, Donzé, & Coleman, 1992). CD 濃度為 0, 0.5, 1, 1.5 和 2 mM. 1.0 mL/min
檢測器 UV λ306 nm
儀器 API 3000 MS/MS 系統 (Applied Biosystems/MDS Sciex, Foster City, CA, USA) 包括 Agilent 1100 HPLC儀 (Agilent Technologies, Wilmington, DE, USA), 及 Analyst™ 1.4.2 軟件.
色譜柱 Luna 3 μm C18 (2) 100 Å 30 mm × 2.0 mm 色譜柱 (Phenomenex, Torrance, CA, USA).
流動相 A: 5 mM 醋酸銨, 水-異丙酮 (98: 2, v/v), B: 甲醇-異丙酮 (98: 2, v/v), A: B = 9: 1 0.5 min, A: B = 5: 95 5 min, 0.25 mL/min
檢測器 負離子模式, 多反應檢測模式 (MRM), 離子噴霧電壓: −3000 V, 碰撞能: -30 V.
樣品製備
方法一
Model GS10A2 多重螺旋星式離心 (Beijing Institute of New Technology Application, Beijing, China) 包括 PTFE 多層線圈 110 m × 1.6 mm I.D. 總容量 230 ml.
乙酸乙酯-乙醇-水 (10: 1: 10, v/v), 容量比例 70: 1: 70.
2.0 mL/min, 80 rpm
UV λ254 nm
參考文獻
[1] Ji, M., Li, Q., Ji, H., & Lou, H. (2014). "Investigation of the distribution and season regularity of resveratrol in Vitis amurensis via HPLC-DAD-MS/MS." Food Chemistry, 142(0), 61-65. doi: http://dx.doi.org/10.1016/j.foodchem.2013.06.131
[2] López-Nicolás, J. M., & García-Carmona, F. (2008). "Rapid, simple and sensitive determination of the apparent formation constants of trans-resveratrol complexes with natural cyclodextrins in aqueous medium using HPLC." Food Chemistry, 109(4), 868-875. doi: http://dx.doi.org/10.1016/j.foodchem.2008.01.022
[3] Muzzio, M., Huang, Z., Hu, S.-C., Johnson, W. D., McCormick, D. L., & Kapetanovic, I. M. (2012). "Determination of resveratrol and its sulfate and glucuronide metabolites in plasma by LC-MS and their pharmacokinetics in dogs." Journal of Pharmaceutical and Biomedical Analysis, 59(0), 201-208. doi: http://dx.doi.org/10.1016/j.jpba.2011.10.023
[4] Feng, Y., Wang, X.-p., Yang, S.-g., Wang, Y.-j., Zhang, X., Du, X.-t., ... Liu, R.-t. (2009). "Resveratrol inhibits beta-amyloid oligomeric cytotoxicity but does not prevent oligomer formation." NeuroToxicology, 30(6), 986-995. doi: http://dx.doi.org/10.1016/j.neuro.2009.08.013
[5] Soylemez, S., Gurdal, H., Sepici, A., & Akar, F. (2008). "The effect of long-term resveratrol treatment on relaxation to estrogen in aortae from male and female rats: Role of nitric oxide and superoxide." Vascular Pharmacology, 49(2-3), 97-105. doi: http://dx.doi.org/10.1016/j.vph.2008.06.006
[6] Belguendouz, L., Fremont, L., & Linard, A. (1997). "Resveratrol inhibits metal ion-dependent and independent peroxidation of porcine low-density lipoproteins." Biochemical Pharmacology, 53(9), 1347-1355. doi: http://dx.doi.org/10.1016/S0006-2952(96)00820-9
[7] Notas, G., Nifli, A.-P., Kampa, M., Vercauteren, J., Kouroumalis, E., & Castanas, E. (2006). "Resveratrol exerts its antiproliferative effect on HepG2 hepatocellular carcinoma cells, by inducing cell cycle arrest, and NOS activation." Biochimica et Biophysica Acta (BBA) - General Subjects, 1760(11), 1657-1666. doi: http://dx.doi.org/10.1016/j.bbagen.2006.09.010
[8] Wong, D. H., Villanueva, J. A., Cress, A. B., Sokalska, A., Ortega, I., & Duleba, A. J. (2011). "Resveratrol inhibits the mevalonate pathway and potentiates the antiproliferative effects of simvastatin in rat theca-interstitial cells." Fertility and Sterility, 96(5), 1252-1258. doi: http://dx.doi.org/10.1016/j.fertnstert.2011.08.010
[9] Chen, L., Han, Y., Yang, F., & Zhang, T. (2001). "High-speed counter-current chromatography separation and purification of resveratrol and piceid from Polygonum cuspidatum." Journal of Chromatography A, 907(1-2), 343-346. doi: http://dx.doi.org/10.1016/S0021-9673(00)00960-2
[10] Chen, H., Tuck, T., Ji, X., Zhou, X., Kelly, G., Cuerrier, A., & Zhang, J. (2013). "Quality Assessment of Japanese Knotweed (Fallopia japonica) Grown on Prince Edward Island as a Source of Resveratrol." J. Agric. Food Chem., 61(26), 6383-6392. doi: 10.1021/jf4019239
[11] Glavnik, V., Simonovska, B., Albreht, A., & Vovk, I. (2012). "TLC and HPLC screening of p-coumaric acid, trans-resveratrol, and pterostilbene in bacterial cultures, food supplements, and wine." J. Planar Chromatogr.--Mod. TLC, 25(3), 251-258. doi: 10.1556/JPC.25.2012.3.11

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