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植物來源 |
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異名 |
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生物活性 |
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鑑定 |
熔點 |
117-119°C |
| 旋光度 |
[α]D28±3° (C=3.0, 氯仿) |
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| 分析方法 |
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| 儀器 |
高效矽膠板GF254 |
| 流動相 |
石油醚: 甲酸乙酯: 甲酸 = 15: 5: 1 |
| 檢測器 |
UV λ254 nm |
|
|
|
| 儀器 |
Shimadzu LC-2010 液相色譜 (Shimadzu Corp., Kyoto, Japan) 和 Shimadzu LC 工作軟件 |
| 色譜柱 |
Shimadzu C18 色譜柱 (Phenomenex, 150 × 4.6 mm, 5μm). |
| 流動相 |
甲醇: 去離子水 = 70: 30, 0.8 mL/min |
| 檢測器 |
UV λ280 nm |
|
|
|
| 儀器 |
Agilent 1100 HPLC儀 (Agilent Technologies, Palo Alto, CA, USA), 包括一個二元泵, 自動進樣器, 真空脫氣機, 自動恒溫柱溫箱, UV檢測器. |
| 色譜柱 |
Elite ODS C18 色譜柱 (250 mm × 4.6 mm, 5 μm) |
| 流動相 |
A: 乙腈, B: 水, 1.0 mL/min |
| 檢測器 |
UV λ217 nm |
|
|
|
| 儀器 |
Q TRAPTM 4000 MS/MS 系統, Applied AB Sciex (Foster City, CA, USA) 配備一個 Prominence™ UFLC 系統 (Shimadzu, Kyoto, Japan) |
| 色譜柱 |
Shim-pack XR-ODS 色譜柱 (75 mm × 3.0 mm, 2.2 μm) (Shimadzu, Kyoto, Japan) |
| 流動相 |
A: 0.1% 甲酸水, B: 乙腈, 0.4 mL/min |
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| 樣品製備 |
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方法一 |
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Model TBE-300 HSCCC 儀 (Shanghai Tauto Biological Company, China) 配備三個製備線圈 (PTFE 管徑, 2.6 mm; 總容量, 119 ml) 和 10 ml 樣品回路. |
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正己烷: 甲醇: 水 = 35: 30: 3, v/v |
|
|
1.0 mL/min |
|
|
UV λ254 nm |
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|
| 參考文獻 |
|
[1]
|
Guo, L. Y., et al. (2008). "Anti-inflammatory effects of schisandrin isolated from the fruit of Schisandra chinensis Baill." European Journal of Pharmacology 591(1–3): 293-299. |
|
[2]
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Liu, H., et al. (2012). "Chemical analysis of twelve lignans in the fruit of Schisandra sphenanthera by HPLC–PAD-MS." Phytomedicine 19(13): 1234-1241. |
|
[3]
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Chiu, P. Y., et al. (2008). "Schisandrin B stereoisomers protect against hypoxia/reoxygenation-induced apoptosis and inhibit associated changes in Ca2+-induced mitochondrial permeability transition and mitochondrial membrane potential in H9c2 cardiomyocytes." Life Sciences 82(21–22): 1092-1101. |
|
[4]
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Lam, P. Y., et al. (2011). "Schisandrin B protects against solar irradiation-induced oxidative stress in rat skin tissue." Fitoterapia 82(3): 393-400. |
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[5]
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Ip, S.-P. and K.-M. Ko (1996). "The crucial antioxidant action of schisandrin B in protecting against carbon tetrachloride hepatotoxicity in mice: A comparative study with butylated hydroxytoluene." Biochemical Pharmacology 52(11): 1687-1693. |
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[6]
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LI Li-shun, S. W.-j., WANG Gui-yang (2011). "Identification and Determination of Three Kinds of Schisandra by HPTLC and HPLC." 《Lishizhen Medicine and Materia Medica Research》 22(1008-0805). |
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[7]
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Gu, W., Wei, N., & Wang, Z. (2008). "LC analysis of lignans from Schisandra sphenanthera Rehd. et Wils. Chromatographia", 67(11/12), 979-983. doi: 10.1365/s10337-008-0621-7 |
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[8]
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Lu, T.-L., et al. (2012). "Quality analysis of raw and processed Schisandra chinensis fructus by simultaneous determination of eleven bioactive lignans using RP-HPLC method." J. Food Drug Anal. 20(4): 922-929. |
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[9]
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Wei, B., et al. (2013). "Development of a UFLC–MS/MS method for simultaneous determination of six lignans of Schisandra chinensis (Turcz.) Baill. in rat plasma and its application to a comparative pharmacokinetic study in normal and insomnic rats." Journal of Pharmaceutical and Biomedical Analysis 77(0): 120-127. |
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[10]
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Huang, T., et al. (2005). "Preparative separation and purification of deoxyschisandrin and γ-schisandrin from Schisandra chinensis (Turcz.) Baill by high-speed counter-current chromatography." Journal of Chromatography A 1066(1–2): 239-242. |
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| 連結 |
 中藥材圖像數據庫
藥用植物圖像數據庫
中藥標本數據庫
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