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植物來源 |
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生物活性 |
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鑑定 |
熔點 |
184-185°C |
| 旋光度 |
[α]20D-85.0±3.0° (C=0.1g/100ml, 氯仿) |
1HNMR
|
| 分析方法 |
|
| 儀器 |
HP1100 液相色譜儀或Agilent 1100 液相色譜儀 (Hewlett Packard, Palo Alto, CA, USA), 包括一個四元泵, 在線脫氣機, 恒溫柱, model 0497 進樣閥 (20 μL樣品回路)及一個光電二極管數組檢測器或一個可變波長檢測器 |
| 色譜柱 |
Diamonsil C18 色譜柱 (150 mm × 4.6 mm i.d., 5 μm) |
| 流動相 |
甲醇: 四氫呋喃: 水: 冰醋酸 = 20: 35: 44: 1, v/v/v/v, 1.0 mL/min |
| 檢測器 |
UV λ254 nm |
|
|
|
| 儀器 |
Finnigan Surveyor™ HPLC 儀 (Thermo Electron, San Jose, CA, USA) |
| 色譜柱 |
150 mm × 2.0 mm Shim-pack VP-ODS 分析色譜柱 (Shimadzu, Kyoto, Japan) 保護柱 (Phenomenex, Inc.) |
| 流動相 |
乙腈: 0.05% 氨水: 80: 20, v/v, 0.2 mL/min |
| 檢測器 |
正離子模式, m/z 297 → 251為隱丹參酮, 掃描時間0.2 s位移 |
|
|
|
| 儀器 |
Waters Acquity UPLC 儀, 包括二元溶劑控制器, 進樣控制器, 色譜柱組件, PDA 檢測器, Waters Empower 2 軟件. |
| 色譜柱 |
Acquity UPLC BEH C18 色譜柱 (50 mm × 2.1 mm, 1.7 μm) |
| 流動相 |
A: 水, B: 乙腈 |
| 檢測器 |
UV λ280 nm |
|
| 樣品製備 |
|
方法一 |
|
|
|
|
|
HSCCC (TBE-300), Shenzhen Tauto Biotech, Shanghai, China, 包括三個製備線圈 (管徑 = 2.6 mm, 總體積 = 300 mL), 20 mL 樣品回路, Model S 恒流泵, Model 8823A UV 檢測器, 波長 280 nm, Model 3057 記錄器. |
|
|
溶劑系統 A: 正己烷: 乙醇: 水 = 10: 5.5: 4.5, v/v, B 正己烷: 乙醇: 水 = 10: 7: 3, v/v, 470 min 洗脫, A-B |
|
|
2.0 mL/min, 900rpm |
|
|
UV λ280 nm |
|
|
| 參考文獻 |
|
[1]
|
Song, M., et al. (2007). "Effects of the coexisting diterpenoid tanshinones on the pharmacokinetics of cryptotanshinone and tanshinone IIA in rat." European Journal of Pharmaceutical Sciences 32(4–5): 247-253. |
|
[2]
|
Jin, D.-Z., et al. (2006). "Cryptotanshinone inhibits cyclooxygenase-2 enzyme activity but not its expression." European Journal of Pharmacology 549(1–3): 166-172. |
|
[3]
|
Park, I.-J., et al. (2010). "Cryptotanshinone sensitizes DU145 prostate cancer cells to Fas (APO1/CD95)-mediated apoptosis through Bcl-2 and MAPK regulation." Cancer Letters 298(1): 88-98. |
|
[4]
|
Zhou, Z., et al. (2006). "Cryptotanshinone inhibits endothelin-1 expression and stimulates nitric oxide production in human vascular endothelial cells." Biochimica et Biophysica Acta (BBA) - General Subjects 1760(1): 1-9. |
|
[5]
|
Chiou, W.-F. and M.-J. Don (2007). "Cryptotanshinone inhibits macrophage migration by impeding F-actin polymerization and filopodia extension." Life Sciences 81(2): 109-114. |
|
[6]
|
Jin, Q., et al. "Hepatoprotective effect of cryptotanshinone from Salvia miltiorrhiza in d-galactosamine/lipopolysaccharide-induced fulminant hepatic failure." Phytomedicine (0). |
|
[7]
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Kim, W. S., et al. (2012). "Cryptotanshinone and tanshinone IIA enhance IL-15-induced natural killer cell differentiation." Biochemical and Biophysical Research Communications 425(2): 340-347. |
|
[8]
|
Shi, Z., et al. (2005). "Simultaneous determination of cryptotanshinone, tanshinone I and tanshinone IIA in traditional Chinese medicinal preparations containing Radix salvia miltiorrhiza by HPLC." Journal of Pharmaceutical and Biomedical Analysis 37(3): 481-486. |
|
[9]
|
Hao, H., et al. (2006). "Simultaneous quantification of cryptotanshinone and its active metabolite tanshinone IIA in plasma by liquid chromatography/tandem mass spectrometry (LC–MS/MS)." Journal of Pharmaceutical and Biomedical Analysis 40(2): 382-388. |
|
[10]
|
Wu, W., & Tang, J. (2009). "UPLC simultaneous determination of 5 lipophilic components in Salvia miltiorrhiza Bge." Yaowu Fenxi Zazhi, 29(5), 827-829. |
|
[11]
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Gu, M., et al. (2004). "Identification of major active constituents in the fingerprint of Salvia miltiorrhiza Bunge developed by high-speed counter-current chromatography." Journal of Chromatography A 1041(1–2): 239-243. |
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| 連結 |
 中藥材圖像數據庫
藥用植物圖像數據庫
中藥標本數據庫
|
