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Natural Resources |
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Bioactivities |
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Identification |
Melting point |
108.5°C |
| Optical rotation |
[α]20D-21° (alcohol) |
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| Analytical Method |
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| INSTRUMENT |
20 × 20 silica gel aluminium sheets 60 F254 (Merck, Darmstadt, Germany) |
| MOBILE PHASE |
Chloroform: methanol: ammonia (44: 9: 1) |
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| INSTRUMENT |
LC-6A HPLC pump coupled to an SPD-6AV UV detector (Shimadzu) |
| COLUMN |
C18 column (3.9 × 300 mm, 5 μm) (Novapak) |
| MOBILE PHASE |
A: acetonitrile, B: 5 mM SDS, 40% A, v/v, pH 3.5, 1.1 mL/min |
| DETECTION |
UV λ204 nm |
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| INSTRUMENT |
1100 LC/MSD Series (liquid chromatography-mass selective detector) (Palo Alto, CA, USA) |
| COLUMN |
Chirobiotic V column (250 × 4.6 mm, 5 um) connected to a Chirobiotic V guard column (2 cm × 4.0 mm, 5 um) (Astec, Advanced Separation Technologies Inc., Whippany, NJ, USA) |
| MOBILE PHASE |
methanol: acetic acid: triethylamine (0-25 min, 100: 0.05: 0.04-100: 0.05: 0.1, v/v/v, 1 mL/min) |
| DETECTION |
atmospheric pressure chemical ionization interface (APCI), positive mode, drying gas temperature: 350°C, vaporizer temperature: 210°C, capillary voltage: 4000 V, fragmentor voltage: 70 V |
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| INSTRUMENT |
A GC Varian mod. 3300/3400 |
| COLUMN |
Capillary column J. & W. OV1 (15 × 0.25 mm, film thickness 0.25 μm), 100°C for 1 min programmed to 280°C for 30 min. Manifold: 220°C, injector: 280°C, transfer line: 290°C |
| DETECTION |
Finnigan Mat mod. ITS 40 Mass Spectrometer with ion trap detector in full scan (40-500 amu) |
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| Reference |
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[1]
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Pramod, K. K., et al. (2010). "Biochemical and structural characterization of recombinant hyoscyamine 6β-hydroxylase from Datura metel L." Plant Physiology and Biochemistry 48(12): 966-970. |
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[2]
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el Jaber-Vazdekis, N., et al. (2009). "Cloning, characterization and analysis of expression profiles of a cDNA encoding a hyoscyamine 6β-hydroxylase (H6H) from Atropa baetica Willk." Plant Physiology and Biochemistry 47(1): 20-25. |
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[3]
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Ghelardini, C., et al. (2000). "Pro-cognitive activity induced in the rat by low doses of R-(+)-hyoscyamine." Fitoterapia 71, Supplement 1(0): S124-S130. |
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[4]
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Ghelardini, C., et al. (1997). "Stereoselective Increase in Cholinergic Transmission by R-(+)-hyoscyamine." Neuropharmacology 36(3): 281-294. |
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[5]
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Rozear, M., et al. (1968). "Effects of intracerebroventricular L-hyoscyamine, ethybenztropine and procaine on cardiac arrhythmias induced in dogs by pentylenetetrazol, picrotoxin or deslanoside." International Journal of Neuropharmacology 7(1): 1-IN3. |
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[6]
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Rocha, P., et al. (2002). "Functional expression of tropinone reductase I (trI) and hyoscyamine-6β-hydroxylase (h6h) from Hyoscyamus niger in Nicotiana tabacum." Plant Science 162(6): 905-913. |
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[7]
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Siluk, D., et al. (2007). "HPLC-atmospheric pressure chemical ionization mass spectrometric method for enantioselective determination of R,S-propranolol and R,S-hyoscyamine in human plasma." Journal of Chromatography B 859(2): 213-221. |
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[8]
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Miraldi, E., et al. (2001). "Distribution of hyoscyamine and scopolamine in Datura stramonium." Fitoterapia 72(6): 644-648. |
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| Link to |
 Chinese Medicinal Material Images Database
Medicinal Plant Images Database
Chinese Medicine Specimen Database
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