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植物來源 |
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生物活性 |
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鑑定 |
熔點 |
212°C |
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| 分析方法 |
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| 儀器 |
矽膠G板 |
| 流動相 |
氯仿: 甲醇: 水 = 45: 17: 5, v/v/v |
| 檢測器 |
UV365 nm |
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| 儀器 |
LC-10ATVP HPLC 泵 (Shimazdu, Japan) 配備 SPD-10AVP UV檢測器 (Shimazdu, Japan) |
| 色譜柱 |
Diamonsil-C18 色譜柱 250 mm × 4.6 mm I.D. (5 µm C18 矽膠, Dikma Company, Beijing, China) |
| 流動相 |
冰醋酸: 水: 乙腈 = 2.3 : 75.7: 22 (v/v/v); 流速: 1.0 mL/min |
| 檢測器 |
UV λ276 nm |
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| 儀器 |
HP1100 色譜儀 Agilent Technologies, USA |
| 色譜柱 |
C18 色譜柱 (250 mm × 4.6 mm × 5 µm, Agilent Technologies, USA) |
| 流動相 |
A: 乙腈, B: 0.5% 乙酸溶液, 0-11 min 20-40% B, 11-19 min 40% B, 19-20 min 40-20% B; 流速: 1.0 mL/min. |
| 檢測器 |
UV λ276 nm |
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| 儀器 |
Agilent 1200LC 色譜儀, U.S.A. |
| 色譜柱 |
ODS 色譜柱 (250 mm × 4.6 mm, 5 µm, Agilent, U.S.A.), 25°C |
| 流動相 |
甲醇: 水 = 45: 55, v/v; 1.0 mL/min |
| 檢測器 |
UV λ276 nm |
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| 儀器 |
Hewlett-Packard (Waldbronn, Germany) 系統配備 HP1100 series 二元泵, HP1100 series 光電二極管數組檢測器和 HP1100 series 自動進樣器 (5 µl) |
| 色譜柱 |
Wako Wakosil-II 5C18 AR 反相色譜柱 (粒徑5 µm, 150 × 4.6 mm i.d.) |
| 流動相 |
0.01% (v/v) 硫酸: 乙腈 = 0 min, 90: 10; 10 min, 88: 12; 22 min, 70: 30; 30 min, 30: 70; 流速: 1.0 mL/min |
| 檢測器 |
UV λ245 nm |
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| 儀器 |
Waters HPLC 系統包括一個泵 (Model 1525), 自動進樣器 (Model 717 plus), UV檢測器 (Waters 2487 Dual λ 吸收光譜檢測器) |
| 色譜柱 |
Kromasil-C18 色譜柱 (5 µm, 4.6 × 250 mm, Akzol Nobel, Sweden) |
| 流動相 |
甲醇: 水: 乙酸 = 65: 35: 2, v/v/v; 流速: 1.0 mL/min |
| 檢測器 |
UV λ246 nm |
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| 樣品製備 |
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方法一 |
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150.0 mg PVPB 置入空的聚丙烯墨盒 (Alltech, Deerfield, IL, USA) 預先準備乙醇. 乾燥, 打粉, 1.0 g 樣品用 25.0 mL 甲醇提取 4 小時. SPE 墨盒上樣 0.2 mL. 3.0 mL 乙腈和甲醇: 水沖洗 (75: 25, v/v). 甲醇和甲醇: 乙酸 (90: 10, v/v) 洗脫. |
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方法二 |
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1000 g 樣品用去離子水回流提取 (3 × 3 L) 重複三次 (1 hr). 合併, 真空濃縮得 340 g 提取物, 過大孔吸附樹脂 D101 柱 (10 × 80 cm, Naikai Chemical Co., China), 洗脫, 去離子水 (1 L), 50% 乙醇 (3 L), 95% 乙醇 (3 L) 得三個餾分: 水餾分 (AE1, 122 g), 50% 乙醇餾分 (AE2, 26.8 g), 95% 乙醇餾分 (AE3, 8.83 g). AE2 過矽膠柱 (500 g, 5 × 50 cm, 200 mesh, Qingdao Haiyang Chemical Co., China), 氯仿-MeOH 洗脫 (4: 1, 3: 1, 2: 1, 1: 1, 300 ml), 得到 4 餾分 (sfr): sfr.1 至 sfr.4. 分餾分 3 過 ODS 矽膠柱 (1 × 30 cm, 100 mesh, Fuji Silysia Chemical Ltd. Japan), 洗脫 MeOH- 水, 10% 至 70%, 10% 增量, 每次100 ml. 30% MeOH 洗脫物中得目標化合物 (289 mg) |
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| 參考文獻 |
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[1]
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Wang, W., et al. (2008). "Antidepressant-like effects of liquiritin and isoliquiritin from Glycyrrhiza uralensis in the forced swimming test and tail suspension test in mice." Progress in Neuro-Psychopharmacology and Biological Psychiatry 32(5): 1179-1184. |
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[2]
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Ammar, N. M., et al. (2012). "Phytochemical and clinical studies of the bioactive extract of Glycyrrhiza glabra L. Family Leguminosae." Int. J. Phytomed. 4(3): 429-436, 428pp. |
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[3]
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Zhao, Z., et al. (2008). "Antidepressant-like effect of liquiritin from Glycyrrhiza uralensis in chronic variable stress induced depression model rats." Behavioural Brain Research 194(1): 108-113. |
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[4]
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Ohno, H., et al. (2013). "Evaluation of cytotoxiciy and tumor-specificity of licorice flavonoids based on chemical structure." Anticancer Res. 33(8): 3061-3068. |
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[5]
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Zhu, X., et al. (2006). Xuefuzhuyu capsules containing Chinese medicines and its quality control, Tianjin Hongrentang Pharmaceutical Co., Ltd., Peop. Rep. China. 25pp. |
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[6]
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Cong, J. and B. Lin (2007). "Separation of Liquiritin by simulated moving bed chromatography." Journal of Chromatography A 1145(1-2): 190-194. |
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[7]
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Sun, C., et al. (2007). "Separation of glycyrrhizic acid and liquiritin from licorice root by aqueous nonionic surfactant mediated extraction." Colloids and Surfaces A: Physicochemical and Engineering Aspects 305(1-3): 42-47. |
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[8]
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Wang, S., et al. (2013). "Facile optimization for chromatographic separation of liquiritin and liquiritigenin." Journal of Chromatography A 1282(0): 167-171. |
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[9]
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Okamura, N., et al. (1999). "Simultaneous high-performance liquid chromatographic determination of puerarin, daidzin, paeoniflorin, liquiritin, cinnamic acid, cinnamaldehyde and glycyrrhizin in Kampo medicines." Journal of Pharmaceutical and Biomedical Analysis 19(3-4): 603-612. |
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[10]
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Yang, L., et al. (2013). "Development of sample preparation method for isoliquiritigenin, liquiritin, and glycyrrhizic acid analysis in licorice by ionic liquids-ultrasound based extraction and high-performance liquid chromatography detection." Food Chemistry 138(1): 173-179. |
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[11]
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Bi, W., et al. (2010). "Solid-phase extraction of liquiritin and glycyrrhizin from licorice using porous alkyl-pyridinium polymer sorbent." Phytochemical Analysis 21(5): 496-501. |
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| 連結 |
 中藥材圖像數據庫
藥用植物圖像數據庫
中藥標本數據庫
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