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植物来源 |
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异名 |
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生物活性 |
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鉴定 |
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| 分析方法 |
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| 仪器 |
硅胶G板 |
| 流动相 |
苯: 乙酸乙酯: 甲酸 =5: 5: 2 |
| 检测器 |
UV λ365 nm |
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| 仪器 |
SHIMADAZU LC-10A HPLC |
| 色谱柱 |
Spherigel C18 色谱柱, 5Lm, 4.6 x 150 mm, 30°C |
| 流动相 |
乙腈: 水 = 17: 83, 1.0 mL/min |
| 检测器 |
UV λ320 nm |
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| 仪器 |
Shimadzu LC-2010C HT 系统及 Shimadzu HPLC 工作站 (Shimadzu, Japan) |
| 色谱柱 |
Shimadzu VP-ODS 色谱柱 (150 mm × 4.6 mm I.D., 5 μm), 室温 |
| 流动相 |
A: 甲醇, B: 水, 0-60 min 5-100% A |
| 检测器 |
UV λ254 nm |
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| 仪器 |
Waters Acquity™ Ultra Performance LC 系统 (Waters, USA) |
| 色谱柱 |
ACQUITY UPLC BEH C18 色谱柱 (2.1 × 100 mm, 1.7 µm, UK), 40°C |
| 流动相 |
A: 0.1% v/v甲酸水, B: 乙腈, 0-0.5 min 1% B, 0.5-10 min 1-20% B 10-12 min, 20-90% B 12-15 min 90-1% B, and 15-16 min 1% B, 0.5 mL/min |
| 检测器 |
Waters Xevo™ QTof MS (Waters), 一个四级杆及正交加速 TOF-MS (Waters), 包括一个 LockSpray™ 接口及一个ESI 源. 雾化器及辅助气体: 氮气, 碰撞气体: 氩气, 负离子模式, ESI 毛细管电压: 2.5 kV, 源及反溶剂: 120 and 450°C, 脱溶剂及锥气: 700 和30 L/h.采样锥: 40 V, 提取锥: 4.0 V, lockspray 毛细管: 2.0 kV.碰撞能量: 30-45 eV. |
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| 样品制备 |
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方法一 |
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5L 95% 乙醇提取样品成浓缩浆, 超声溶解于 300 ml 水并用等量乙醚和正丁醇分段三次。正丁醇上样 D-101 大孔树脂柱 (35 cm × 3.4 cm, 柱容积170 ml) 并用1700 ml 纯水和 3400 ml 30% 乙醇洗脱。乙醇部分备用于分离。 |
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TBE-300 高速逆流色谱 (Tauto Biotechnique, Shanghai, China) 及三个多层线圈分离柱 (管 I.D. = 1.5 mm, 总体积 = 300 ml) 及一个 20 ml 进样环路及一个 S-1007 恒流泵 (Shenyitong Tech & Exploitation, Beijing, China), 一个 Model 8823B-UV 监视器 (Bingdayingchuang Sci & Tech, Beijing, China). 资料由 model N2000 色谱工作站 (Zhejiang University, Hangzhou, China) 收集 |
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乙酸乙酯: 甲醇: 水 = 50: 1: 50, v/v/v, 流动相: 下相 |
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2.0 mL/min, 800 rpm |
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Shimadzu LC-2010C HT 系统和 Shimadzu HPLC 工作站 (Shimadzu, Japan) 及 Shimadzu VP-ODS 色谱柱 (150 mm × 4.6 mm I.D., 5 μm) 室温, A: 甲醇, B: 乙腈, 0-60 min 5-100% A, UV λ254 nm |
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| 参考文献 |
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[1]
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Yao, S., et al. (2006). "Preparative isolation and purification of chemical constituents from the root of Polygonum multiflorum by high-speed counter-current chromatography." J Chromatogr A 1115(1-2): 64-71. |
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[2]
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Zhang, L., et al. (2012). "Effect of TSG on nigral dopaminergic transporter (DAT) in MPTP mouse model of Parkinson\'s disease." Shenjing Jiepouxue Zazhi 28(1): 33-37. |
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[3]
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Sun, F.-l., et al. (2011). "Tetrahydroxystilbene glucoside protects human neuroblastoma SH-SY5Y cells against MPP+-induced cytotoxicity." Eur. J. Pharmacol. 660(2-3): 283-290. |
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[4]
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Qin, R., et al. (2011). "Protection by tetrahydroxystilbene glucoside against neurotoxicity induced by MPP+: The involvement of PI3K/Akt pathway activation." Toxicol. Lett. 202(1): 1-7. |
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[5]
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Tao, L., et al. (2011). "TSG attenuated 6-OHDA-induced apoptosis in PC12 cells by the inhibition of ROS." Shenjing Jiepouxue Zazhi 27(1): 19-23. |
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[6]
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Zhang, J. K., et al. (2012). "Protective effect of tetrahydroxystilbene glucoside against hydrogen peroxide-induced dysfunction and oxidative stress in osteoblastic MC3T3-E1 cells." Eur J Pharmacol 689(1-3): 31-37. |
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[7]
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Zhang, S. H., et al. (2009). "Protective effect of tetrahydroxystilbene glucoside on cardiotoxicity induced by doxorubicin in vitro and in vivo." Acta Pharmacol Sin 30(11): 1479-1487. |
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[8]
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Yao, W., et al. (2013). "Proteomic analysis for anti-atherosclerotic effect of tetrahydroxystilbene glucoside in rats." Biomed. Pharmacother. 67(2): 140-145. |
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[9]
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Yao, W., et al. (2013). "Proteomic analysis for anti-atherosclerotic effect of tetrahydroxystilbene glucoside in rats." Biomed Pharmacother 67(2): 140-145. |
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[10]
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Wang, X., et al. (2008). "Protective effects of 2,3,5,4\'-tetrahydroxystilbene-2-O-beta-d-glucoside, an active component of Polygonum multiflorum Thunb, on experimental colitis in mice." Eur J Pharmacol 578(2-3): 339-348. |
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[11]
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Liu, Y., et al. (2012). "Effects of tetrahydroxy-stilbene glycoside on α-synuclein overexpression and ubiquitin-proteasome system in gene-transfected PC12 cells." Zhongguo Yaoxue Zazhi (Beijing, China) 47(1): 34-39. |
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[12]
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Xu, X.-L., et al. (2012). "2,3,4\',5-Tetrahydroxystilbene-2-O-β-D-glucoside Inhibits Proliferation of Vascular Smooth Muscle Cells: Involvement of NO/cGMP/PKG Pathway." Phytother. Res. 26(7): 1068-1074. |
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[13]
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Li, J., et al. (2013). "Effects of tetrahydroxystilbene-2-O-β-D-glucoside on apoptosis and expressions of bcl-2/bax/caspase-3 in HUVECs treated with homocysteine." Zhongguo Bingli Shengli Zazhi 29(4): 743-747. |
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[14]
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Zhou, L., et al. (2012). "Tetrahydroxystilbene glucoside improves the learning and memory of amyloid-β1-42-injected rats and may be connected to synaptic changes in the hippocampus." Can. J. Physiol. Pharmacol. 90(11): 1446-1455. |
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[15]
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Zhang, W., et al. (2009). "Effects of 2,3,4\',5-tetrahydroxystilbene 2-O-β-D-glucoside on vascular endothelial dysfunction in atherogenic-diet rats." Planta Med. 75(11): 1209-1214. |
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[16]
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Hu, Y., et al. (2009). "Quality standard for YiShenyangyuan mixture." Guangdong Yaoxueyuan Xuebao 25(2): 157-159. |
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[17]
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Xie, D., et al. (2003). "Determination of 2,3,5,4\'-tetrahydroxystibene-2-O-β-D-glucoside in Yangxue Bushen oral liquid by HPLC." Guangdong Yaoxueyuan Xuebao 19(4): 332-333, 339. |
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[18]
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Zhao, Y.-Y., et al. (2013). "Pharmacokinetics of 2,3,5,4\'-tetrahydroxystilbene-2-O-β-D-glucoside in rat using ultra-performance LC-quadrupole TOF-MS." J. Sep. Sci. 36(5): 863-871. |
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| 连结 |
 中药材图像数据库
药用植物图像数据库
中药标本数据库
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