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Natural Resources |
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Synonyms |
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Bioactivities |
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Identification |
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| Analytical Method |
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| INSTRUMENT |
Silica Gel G plate |
| MOBILE PHASE |
Benzene: ethyl acetate: Formic acid = 5: 5: 2 |
| DETECTION |
UV λ365 nm |
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| INSTRUMENT |
SHIMADAZU LC-10A HPLC |
| COLUMN |
Spherigel C18 column, 5Lm, 4.6 x 150 mm, 30°C |
| MOBILE PHASE |
Acetonitrile: water = 17: 83, 1.0 mL/min |
| DETECTION |
UV λ320 nm |
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| INSTRUMENT |
Shimadzu LC-2010C HT system and Shimadzu HPLC workstation (Shimadzu, Japan) |
| COLUMN |
Shimadzu VP-ODS column (150 mm × 4.6 mm I.D., 5 μm) at room temperature |
| MOBILE PHASE |
A: methanol, B: water, 0-60 min 5-100% A |
| DETECTION |
UV λ254 nm |
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| INSTRUMENT |
Waters Acquity™ Ultra Performance LC system (Waters, USA) |
| COLUMN |
ACQUITY UPLC BEH C18 column (2.1 × 100 mm, 1.7 µm, UK), 40°C |
| MOBILE PHASE |
A: 0.1% v/v formic acid in water, B: acetonitrile, 0-0.5 min 1% B, 0.5-10 min 1-20% B 10-12 min, 20-90% B 12-15 min 90-1% B, and 15-16 min 1% B, 0.5 mL/min |
| DETECTION |
Waters Xevo™ QTof MS (Waters), a quadrupole and orthogonal acceleration TOF-MS (Waters), which was equipped with a LockSpray™ interface and an ESI source. Nebulizer and auxiliary gas: nitrogen, collision gas: argon, negative ion mode, ESI capillary voltage: 2.5 kV, source and desolvation: 120 and 450°C, desolvation and cone gas: 700 and 30 L/h.The sample cone: 40 V, extraction cone: 4.0 V, lockspray capillary: 2.0 kV. The collision energy: 30-45 eV. |
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| Sample Preparation |
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METHOD 1 |
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Sample extracted with 5 L 95% ethanol to form syrup, and was dissolved in 300 ml of water by sonication and partitioned with ether and n-butanol of equal volume three times. n-butanol was loaded on D-101 macroporous resin column (35 cm × 3.4 cm, the volume of the column was 170 ml) and eluted with 1700 ml of distilled water and 3400 ml of 30% ethanol. The ethanol part is prepared for separation. |
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TBE-300 high-speed counter-current chromatography (Tauto Biotechnique, Shanghai, China) with three multilayer coil separation column connected in series (I.D. of the tubing = 1.5 mm, total volume = 300 ml) and a 20 ml sample loop and a S-1007 constant flow pump (Shenyitong Tech & Exploitation, Beijing, China), a Model 8823B-UV monitor (Bingdayingchuang Sci & Tech, Beijing, China). The data were collected with model N2000 chromatography workstation (Zhejiang University, Hangzhou, China) |
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Ethyl acetate: methanol: water = 50: 1: 50, v/v/v, mobile phase: lower phase |
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2.0 mL/min, 800 rpm |
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Shimadzu LC-2010C HT system and Shimadzu HPLC workstation (Shimadzu, Japan) with Shimadzu VP-ODS column (150 mm × 4.6 mm I.D., 5 μm) at room temperature, A: methanol, B: water, 0-60 min 5-100% A, UV λ254 nm |
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| Reference |
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[1]
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Yao, S., et al. (2006). "Preparative isolation and purification of chemical constituents from the root of Polygonum multiflorum by high-speed counter-current chromatography." J Chromatogr A 1115(1-2): 64-71. |
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[2]
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Zhang, L., et al. (2012). "Effect of TSG on nigral dopaminergic transporter (DAT) in MPTP mouse model of Parkinson\'s disease." Shenjing Jiepouxue Zazhi 28(1): 33-37. |
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[3]
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Sun, F.-l., et al. (2011). "Tetrahydroxystilbene glucoside protects human neuroblastoma SH-SY5Y cells against MPP+-induced cytotoxicity." Eur. J. Pharmacol. 660(2-3): 283-290. |
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[4]
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Qin, R., et al. (2011). "Protection by tetrahydroxystilbene glucoside against neurotoxicity induced by MPP+: The involvement of PI3K/Akt pathway activation." Toxicol. Lett. 202(1): 1-7. |
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[5]
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Tao, L., et al. (2011). "TSG attenuated 6-OHDA-induced apoptosis in PC12 cells by the inhibition of ROS." Shenjing Jiepouxue Zazhi 27(1): 19-23. |
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[6]
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Zhang, J. K., et al. (2012). "Protective effect of tetrahydroxystilbene glucoside against hydrogen peroxide-induced dysfunction and oxidative stress in osteoblastic MC3T3-E1 cells." Eur J Pharmacol 689(1-3): 31-37. |
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[7]
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Zhang, S. H., et al. (2009). "Protective effect of tetrahydroxystilbene glucoside on cardiotoxicity induced by doxorubicin in vitro and in vivo." Acta Pharmacol Sin 30(11): 1479-1487. |
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[8]
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Yao, W., et al. (2013). "Proteomic analysis for anti-atherosclerotic effect of tetrahydroxystilbene glucoside in rats." Biomed. Pharmacother. 67(2): 140-145. |
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[9]
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Yao, W., et al. (2013). "Proteomic analysis for anti-atherosclerotic effect of tetrahydroxystilbene glucoside in rats." Biomed Pharmacother 67(2): 140-145. |
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[10]
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Wang, X., et al. (2008). "Protective effects of 2,3,5,4\'-tetrahydroxystilbene-2-O-beta-d-glucoside, an active component of Polygonum multiflorum Thunb, on experimental colitis in mice." Eur J Pharmacol 578(2-3): 339-348. |
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[11]
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Liu, Y., et al. (2012). "Effects of tetrahydroxy-stilbene glycoside on α-synuclein overexpression and ubiquitin-proteasome system in gene-transfected PC12 cells." Zhongguo Yaoxue Zazhi (Beijing, China) 47(1): 34-39. |
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[12]
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Xu, X.-L., et al. (2012). "2,3,4\',5-Tetrahydroxystilbene-2-O-β-D-glucoside Inhibits Proliferation of Vascular Smooth Muscle Cells: Involvement of NO/cGMP/PKG Pathway." Phytother. Res. 26(7): 1068-1074. |
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[13]
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Li, J., et al. (2013). "Effects of tetrahydroxystilbene-2-O-β-D-glucoside on apoptosis and expressions of bcl-2/bax/caspase-3 in HUVECs treated with homocysteine." Zhongguo Bingli Shengli Zazhi 29(4): 743-747. |
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[14]
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Zhou, L., et al. (2012). "Tetrahydroxystilbene glucoside improves the learning and memory of amyloid-β1-42-injected rats and may be connected to synaptic changes in the hippocampus." Can. J. Physiol. Pharmacol. 90(11): 1446-1455. |
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[15]
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Zhang, W., et al. (2009). "Effects of 2,3,4\',5-tetrahydroxystilbene 2-O-β-D-glucoside on vascular endothelial dysfunction in atherogenic-diet rats." Planta Med. 75(11): 1209-1214. |
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[16]
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Hu, Y., et al. (2009). "Quality standard for YiShenyangyuan mixture." Guangdong Yaoxueyuan Xuebao 25(2): 157-159. |
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[17]
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Xie, D., et al. (2003). "Determination of 2,3,5,4\'-tetrahydroxystibene-2-O-β-D-glucoside in Yangxue Bushen oral liquid by HPLC." Guangdong Yaoxueyuan Xuebao 19(4): 332-333, 339. |
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[18]
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Zhao, Y.-Y., et al. (2013). "Pharmacokinetics of 2,3,5,4\'-tetrahydroxystilbene-2-O-β-D-glucoside in rat using ultra-performance LC-quadrupole TOF-MS." J. Sep. Sci. 36(5): 863-871. |
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 Chinese Medicinal Material Images Database
Medicinal Plant Images Database
Chinese Medicine Specimen Database
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