Name Phytol
Appearance Colorless oil
CAS No. 150-86-7
Formulae
Molecular Weight 296.535
Natural Resources
Bioactivities
Identification
1HNMR
13CNMR
Analytical Method
INSTRUMENT Perkin-Elmer GC Clarus 500 system and Gas chromatograph interfaced to a Mass spectrometer (GC-MS)
COLUMN Elite-I, fused silica capillary column (30 mm x 0.25 mm 1 D x 1μMdf, composed of 100% Dimethyl poly siloxane), Helium gas was used as the carrier gas at constant flow rate 1 mL/min and an injection volume of 2μL was employed (split ratio of 10 : 1).
MOBILE PHASE The oven temperature was programmed from 110°C (isothermal for 2 min), with an increase of 10°C/min, to 200°C, then 5°C/min to 280°C ending with a 9 min isothermal at 280°C
DETECTION Ionizing energy of 70 eV was used. Injector temperature 250°C ; Ion-source temperature 280°C
INSTRUMENT GC clarus 500 Perkin Elmer system comprising a AOC- 20i autosampler and gas chromatograph interfaced to a mass spectrometer (GC-MS) instrument e
COLUMN Elite-1 fused silica capillary column (330 mm x 0.25 mm ID x 1 μm df), composed of 100% Dimethyl poly siloxane, Helium (99.999%) was used as carrier gas at a constant flow of 1 mL/min and an injection volume of 0.5 μl was employed (split ratio of 10: 1)
MOBILE PHASE Oven temperature was programmed from 110°C with an increase of 10°C/min, to 200°C, then 5°C/min to 280°C, ending with a 9 minisothermal at 280°C
DETECTION Injector temperature 250°C; ion-source temperature 280°C.
INSTRUMENT GC CLARUS 500 PerkinElmer system comprising a gas chromatograph interfaced to a mass spectrometer (GC-MS)
COLUMN Elite-1 fused silica capillary column (30 × 0.25 mm ID × 1EM df, composed of 100% Dimethyl poly siloxane), operating in electron impact mode at 70 eV; Helium (99.999%) was used as carrier gas at a constant flow of 1mL/min and an injection volume of 0.5 EI was employed.
MOBILE PHASE The oven temperature was programmed from 110°C (isothermal for 2 min), with an increase of 10°C/min, to 200°C, then 5°C/min to 280°C, ending with a 9 min isothermal at 280°C.
DETECTION Mass spectra were taken at 70 eV; a scan interval of 0.5 s and fragments from 40 to 550 Da.
Sample Preparation
METHOD 1
Samples were saponified by ethanolic solution of potassium hydroxide and the alkali concentration was firstly optimized. The sample was extracted by 1.5 mol/L ethanolic KOH by MAE. 2.0 g of homogenized algae sample. Sample extracts were further clarified by centrifugation at 3500 rpm for 5 min and the supernatant was then re-extracted three times with n-hexane. The organic phase was evaporated to dryness and reconstituted with ethanol prior to HPLC analysis.
A J-type instrument (GS10A; Beijing UE Biotech., China) equipped with a 110 m multilayer coil (1.6 mm I.D.) with a total capacity of 240 mL was utilized for high-speed counter current chromatography.
Hexane: acetonitrile: methanol = 5: 5: 3, v/v/v
2.0 mL/min; 800 rpm
UV λ210 nm
Reference
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[2] Wang, Q., et al. (2013). "GC-MS analysis of the low-polarity chemical constituents from Parochetus communis Buch." Med. Plant 4(9): 50-51, 55.
[3] Tang, B.-q., et al. (2013). "Chemical constituents in leaves of Morus atropurpurea and their α-glucosidase activity." Zhongcaoyao 44(22): 3109-3113.
[4] El-Domiaty, M. M., et al. (2013). "Phytochemical and biological investigation of Lagenaria siceraria (Molina) Standl. cultivated in Egypt." Biosci., Biotechnol. Res. Asia 10(2): 533-550.
[5] Zhong, R. M., et al. (2013). "Antimicrobial activity of Myrica rubra essential oil against five pathogenic food-borne bacteria." Adv. Mater. Res. (Durnten-Zurich, Switz.) 781-784(Advances in Chemical Engineering III): 1646-1651, 1647pp.
[6] Sharma, R., et al. (2014). "The potential of Leucosidea sericea against Propionibacterium acnes." Phytochem. Lett. 7: 124-129.
[7] Feng, C., et al. (2008). "Chemical constituents of medicinal mangrove plant Hibiscus tiliaceus." Haiyang Kexue 32(9): 57-60.
[8] Yang, H.-k., et al. (2013). "An analysis on the constituents of the volatile oil from different parts of Ardisia mamillata." Jiangxi Nongye Daxue Xuebao 35(5): 993-998.
[9] Wei, H., et al. (2013). "Chemical constituents in leaves of Cyclosorus parasiticus." Zhongcaoyao 44(17): 2354-2357.
[10] Costa, J. P., et al. (2014). "Anxiolytic-like effects of phytol: Possible involvement of GABAergic transmission." Brain Res. 1547: 34-42.
[11] Costa, J. P., et al. (2012). "Anticonvulsant effect of phytol in a pilocarpine model in mice." Neurosci. Lett. 523(2): 115-118.
[12] Saikia, D., et al. (2010). "Antitubercular potential of some semisynthetic analogues of phytol." Bioorg. Med. Chem. Lett. 20(2): 508-512.
[13] Goto, T., et al. (2005). "Phytol directly activates peroxisome proliferator-activated receptor α (PPARα) and regulates gene expression involved in lipid metabolism in PPARα-expressing HepG2 hepatocytes." Biochemical and Biophysical Research Communications 337(2): 440-445.
[14] Jegadeeswari, P., et al. (2012). "GC-MS analysis of bioactive components of Aristolochia krysagathra (Aristolochiaceae)." J. Curr. Chem. Pharm. Sci. 2(4): 226-232.
[15] Kulandai Therese, N., et al. (2012). "GC-MS analysis of bioactive constituents of Hedyotis leschenaultiana DC (Rubiaceae)." Int. J. Appl. Biol. Pharm. Technol. 3(4): 159-164.
[16] Paranthaman, R., et al. (2012). "GC-MS analysis of phytochemicals and simultaneous determination of flavonoids in Amaranthus caudatus (Sirukeerai) by RP-HPLC." J. Anal. Bioanal. Tech. 3(5): 1000147/1000141-1000147/1000144.
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