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植物来源 |
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生物活性 |
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|
鉴定 |
熔点 |
223-226°C |
| 旋亮度 |
[α]25D-40.6 |
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|
| 分析方法 |
|
| 仪器 |
P/ACE 5500 (Beckman, Fullerton, CA, USA) |
| 色谱柱 |
Bare 硅胶融合毛细管 (Polymicro Technologies, AZ, USA), 27 cm (有效长度 20 cm) × 50 μm I.D. |
| 流动相 |
20 mM 硼酸钠缓冲液 (pH 8.5) 含300 mM SDS |
| 检测器 |
UV λ210 nm |
|
|
|
| 仪器 |
Nanospace SI-2/3001 泵 (Shiseido, Tokyo, Japan) |
| 色谱柱 |
Capcell Pak C18 MG柱 (4.6 mm × 250 mm, 5 μm, Shiseido, Tokyo, Japan) |
| 流动相 |
10 mM 磷酸钠缓冲液 (pH 3.1) 含25% 甲醇或 8.5% 乙腈, 1.2 mL/min |
| 检测器 |
Nanospace SI-2/3002 UV 检测器, UV λ214 nm |
|
|
|
| 色谱柱 |
反相色谱柱 (Zorbax 加长 C18 柱, 3.0 mm × 100 mm i.d., 3.5 μm, Agilent Technologies) 包括一个保护柱 (cartridge 2.1 mm × 12.5 mm, 5 μm, Agilent Technologies), 40°C |
| 流动相 |
10 mM 磷酸钠缓冲液 (pH 3.1) 含30% 甲醇, 0.2 mL/min |
| 检测器 |
喷雾源电压: 4.5 kV, 毛细管电压: 20 V, 加热毛细管温度: 250°C |
|
| 样品制备 |
|
方法一 |
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|
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40-L 色谱柱, 平均 17 mm i.d. 三线圈盘绕的 Teflon 管 |
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固定相: 水, 流动相: 乙酸乙脂-1-丁醇 (1: 2) 饱和水溶液 |
|
|
50.0 mL/min |
|
|
UV λ254 nm |
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|
| 参考文献 |
|
[1]
|
Koo, J.-Y., et al. (2005). "Quantitative determination of amygdalin epimers from armeniacae semen by liquid chromatography." Journal of Chromatography B 814(1): 69-73. |
|
[2]
|
Nout, M. J. R., et al. (1995). "Microbial degradation of amygdalin of bitter apricot seeds (Prunus armeniaca)." International Journal of Food Microbiology 24(3): 407-412. |
|
[3]
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Du, Q., et al. (2005). "Semi-industrial isolation of salicin and amygdalin from plant extracts using slow rotary counter-current chromatography." Journal of Chromatography A 1074(1-2): 43-46. |
|
[4]
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Zhou, C., et al. (2012). "Enhancement of amygdalin activated with β-d-glucosidase on HepG2 cells proliferation and apoptosis." Carbohydrate Polymers 90(1): 516-523. |
|
[5]
|
Rajaei, F., et al. (2013). "The effects of amygdalin on the morphologic structure of mouse cerebellar cortex." Maj. Danishgah-i Ulum-i Pizishki-i Shahrekord 15(3): 26-32. |
|
[6]
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Nie, Z., et al. (2013). "Bladder cancer cells apoptosis induced by amygdalin following specific activation by β-glucosidase." Xiandai Miniao Waike Zazhi 18(2): 113-116. |
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[7]
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Guo, J., et al. (2013). "Amygdalin inhibits renal fibrosis in chronic kidney disease." Mol. Med. Rep. 7(5): 1453-1457. |
|
[8]
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Kang, S. H., et al. (2000). "Micellar electrokinetic chromatography for the analysis of d-amygdalin and its epimer in apricot kernel." Journal of Chromatography A 866(2): 253-259. |
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[9]
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Ge, B. Y., et al. (2007). "Identification of amygdalin and its major metabolites in rat urine by LC-MS." Journal of Chromatography B 857(2): 281-286. |
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| 连结 |
 中药材图像数据库
药用植物图像数据库
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