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Natural Resources |
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Bioactivities |
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Identification |
Melting point |
223-226°C |
| Optical rotation |
[α]25D-40.6 |
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| Analytical Method |
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| INSTRUMENT |
P/ACE 5500 (Beckman, Fullerton, CA, USA) |
| COLUMN |
Bare fused-silica capillary (Polymicro Technologies, AZ, USA) of 27 cm (effective length 20 cm) × 50 μm I.D. |
| MOBILE PHASE |
20 mM sodium borate buffer (pH 8.5) containing 300 mM SDS |
| DETECTION |
UV λ210 nm |
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| INSTRUMENT |
Nanospace SI-2/3001 pump (Shiseido, Tokyo, Japan) |
| COLUMN |
Capcell Pak C18 MG (4.6 mm × 250 mm, 5 μm, Shiseido, Tokyo, Japan) |
| MOBILE PHASE |
10 mM sodium phosphate buffer (pH 3.1) containing 25% methanol or 8.5% acetonitrile, 1.2 mL/min |
| DETECTION |
Nanospace SI-2/3002 UV detector, UV λ214 nm |
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| COLUMN |
Reversed-phase column (Zorbax extend C18, 3.0 mm × 100 mm i.d., 3.5 μm, Agilent Technologies) was connected to a guard column (cartridge 2.1 mm × 12.5 mm, 5 μm, Agilent Technologies), 40°C |
| MOBILE PHASE |
10 mM sodium phosphate buffer (pH 3.1) containing 30% methanol, 0.2 mL/min |
| DETECTION |
Source spray voltage: 4.5 kV, capillary voltage: 20 V, heated capillary temperature: 250°C |
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| Sample Preparation |
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METHOD 1 |
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CCCwith 40-L column three-layer coils 17 mm average i.d. convoluted Teflon tubing |
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Stationary phase: water, Mobile phase: water saturated by ethyl acetate-1-butanol (1: 2) |
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50.0 mL/min |
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UV λ254 nm |
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| Reference |
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[1]
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Koo, J.-Y., et al. (2005). "Quantitative determination of amygdalin epimers from armeniacae semen by liquid chromatography." Journal of Chromatography B 814(1): 69-73. |
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[2]
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Nout, M. J. R., et al. (1995). "Microbial degradation of amygdalin of bitter apricot seeds (Prunus armeniaca)." International Journal of Food Microbiology 24(3): 407-412. |
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[3]
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Du, Q., et al. (2005). "Semi-industrial isolation of salicin and amygdalin from plant extracts using slow rotary counter-current chromatography." Journal of Chromatography A 1074(1-2): 43-46. |
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[4]
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Zhou, C., et al. (2012). "Enhancement of amygdalin activated with β-d-glucosidase on HepG2 cells proliferation and apoptosis." Carbohydrate Polymers 90(1): 516-523. |
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[5]
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Rajaei, F., et al. (2013). "The effects of amygdalin on the morphologic structure of mouse cerebellar cortex." Maj. Danishgah-i Ulum-i Pizishki-i Shahrekord 15(3): 26-32. |
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[6]
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Nie, Z., et al. (2013). "Bladder cancer cells apoptosis induced by amygdalin following specific activation by β-glucosidase." Xiandai Miniao Waike Zazhi 18(2): 113-116. |
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[7]
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Guo, J., et al. (2013). "Amygdalin inhibits renal fibrosis in chronic kidney disease." Mol. Med. Rep. 7(5): 1453-1457. |
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[8]
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Kang, S. H., et al. (2000). "Micellar electrokinetic chromatography for the analysis of d-amygdalin and its epimer in apricot kernel." Journal of Chromatography A 866(2): 253-259. |
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[9]
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Ge, B. Y., et al. (2007). "Identification of amygdalin and its major metabolites in rat urine by LC-MS." Journal of Chromatography B 857(2): 281-286. |
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| Link to |
 Chinese Medicinal Material Images Database
Medicinal Plant Images Database
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